{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T14:14:25Z","timestamp":1791036865055,"version":"4.1.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2000,2,1]],"date-time":"2000-02-01T00:00:00Z","timestamp":949363200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2000,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            \u201c\n            <jats:italic>Tropheryma whippelii<\/jats:italic>\n            \u201d-associated infections are usually confirmed histopathologically by using light microscopy. PCR assays targeting the 16S rRNA gene (16S rDNA) of \u201c\n            <jats:italic>T. whippelii<\/jats:italic>\n            \u201d are increasingly being applied for this purpose. Compared to microscopic analysis, PCR seems to be more sensitive, as indicated by the fact that several cases of Whipple's disease with negative histopathological findings but positive PCR results have been reported. Considering the lack of pathognomonic clinical features for this disease and the fact that \u201c\n            <jats:italic>T. whippelii<\/jats:italic>\n            \u201d DNA has repeatedly been found in patients without clinical Whipple's disease, such PCR results should be confirmed by additional tests. We have, therefore, evaluated a \u201c\n            <jats:italic>T. whippelii<\/jats:italic>\n            \u201d-specific nested PCR targeting domain III of the 23S rDNA with 41 clinical specimens known to contain \u201c\n            <jats:italic>T. whippelii<\/jats:italic>\n            \u201d 16S rDNA. All of these specimens were also positive for \u201c\n            <jats:italic>T. whippelii<\/jats:italic>\n            \u201d 23S rDNA. The specificity of the test was shown by sequencing of the amplicons and by the absence of amplicons in 38 negative controls. We consider this PCR test to be a suitable tool for confirming the presence of \u201c\n            <jats:italic>T. whippelii<\/jats:italic>\n            \u201d DNA in specimens with inconclusive histopathological findings. The information derived from sequencing of the partial \u201c\n            <jats:italic>T. whippelii<\/jats:italic>\n            \u201d 23S rDNA was then combined with our recent data of the 16S-23S rDNA spacer region of this organism. Overall, four different rDNA types are recognized in our proposed classification system for molecular variants of \u201c\n            <jats:italic>T. whippelii<\/jats:italic>\n            .\u201d This preliminary scheme may provide a basis for further epidemiological and clinical studies with \u201c\n            <jats:italic>T. whippelii<\/jats:italic>\n            \u201d and associated diseases.\n          <\/jats:p>","DOI":"10.1128\/jcm.38.2.595-599.2000","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:52:36Z","timestamp":1577814756000},"page":"595-599","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["Evaluation of a Specific Nested PCR Targeting Domain III of the 23S rRNA Gene of \u201c\n            <i>Tropheryma whippelii<\/i>\n            \u201d and Proposal of a Classification System for Its Molecular Variants"],"prefix":"10.1128","volume":"38","author":[{"given":"Hans Peter","family":"Hinrikson","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology, University of Zu\u0308rich, CH-8028 Zu\u0308rich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrizio","family":"Dutly","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology, University of Zu\u0308rich, CH-8028 Zu\u0308rich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Altwegg","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology, University of Zu\u0308rich, CH-8028 Zu\u0308rich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"1495","article-title":"Spondylodiscitis caused by Tropheryma whippelii","volume":"126","author":"Altwegg M.","year":"1996","unstructured":"Altwegg M. Fleisch-Marx A. Goldenberger D. Hailemariam S. Schaffner A. Kissling R. Spondylodiscitis caused by Tropheryma whippelii.Schweiz. Med. Wochenschr.126199614951499","journal-title":"Schweiz. Med. Wochenschr."},{"key":"e_1_3_2_3_2","first-page":"399","article-title":"Relapsing Whipple's disease presenting with hypopituitarism","volume":"50","author":"Br\u00e4ndle M.","year":"1999","unstructured":"Br\u00e4ndle M. Ammann P. Spinas G. A. Dutly F. Galeazzi R. L. Schmid C. Altwegg M. Relapsing Whipple's disease presenting with hypopituitarism.Clin. Endocrinol.501999399403","journal-title":"Clin. Endocrinol."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)90505-X"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-46-4-340"},{"key":"e_1_3_2_6_2","unstructured":"Dobbins\nW. O.\nIII.\nWhipple's disease.\n1987\nCharles C. Thomas\nSpringfield Ill"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199502093320611"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)01776-6"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-42-1-166"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.11.2733-2739.1997"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(94)92176-8"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.1.152-156.1999"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-49-4-1701"},{"key":"e_1_3_2_14_2","unstructured":"Hinrikson H. P. F. Dutly and M. Altwegg. Analysis of the actinobacterial insertion in domain III of the 235 ribosomal RNA gene of uncultured variants of the bacterium associated with Whipple's disease using broad-range and \u201cTropheryma whippelii\u201d-specific PCR. Int. J. Syst. Bacteriol. in press."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-46-4-1078"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/qjmed\/90.12.765"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1136\/gut.40.3.425"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-126-7-199704010-00004"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199207303270501"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199502093320604"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1086\/514089"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-44-4-846"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1053\/gast.1996.v110.pm8964398"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1053\/gast.1997.v113.pm9247461"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)90545-Z"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.38.2.595-599.2000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.38.2.595-599.2000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T00:25:05Z","timestamp":1646439905000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.38.2.595-599.2000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,2]]},"references-count":24,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2000,2]]}},"alternative-id":["10.1128\/JCM.38.2.595-599.2000"],"URL":"https:\/\/doi.org\/10.1128\/jcm.38.2.595-599.2000","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,2]]},"assertion":[{"value":"1999-06-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-11-04","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-02-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}