{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,14]],"date-time":"2025-11-14T17:27:55Z","timestamp":1763141275469},"reference-count":41,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2000,2,1]],"date-time":"2000-02-01T00:00:00Z","timestamp":949363200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2000,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A new selective medium (CNA-P) that reduces or eliminates the inhibitory activity of bacteriocin-producing\n            <jats:italic>Streptococcus salivarius<\/jats:italic>\n            against \u03b2-hemolytic streptococci has been developed and compared with sheep blood agar (SBA) for the sensitive detection of small numbers of \u03b2-hemolytic streptococci in clinical specimens. CNA-P has as its basis a commercial medium (Difco Columbia CNA agar) supplemented with 5% (vol\/vol) sheep blood, and the CNA is further modified by addition of 100 mM PIPES buffer [piperazine-\n            <jats:italic>N<\/jats:italic>\n            ,\n            <jats:italic>N<\/jats:italic>\n            \u2032-bis(2-ethanesulfonic acid)] (pH 7.5) to maintain cultures at an alkaline pH during incubation. CNA-P was shown to inhibit the production and\/or release of four different types of\n            <jats:italic>S. salivarius<\/jats:italic>\n            bacteriocins or bacteriocin-like inhibitory molecules. The efficacies of CNA-P and SBA for detection of \u03b2-hemolytic streptococci in 1,352 pharyngeal samples from 376 children were compared. The \u03b2-hemolytic streptococcal isolates recovered from the samples included 314 group A (\n            <jats:italic>S. pyogenes<\/jats:italic>\n            ), 61 group G, 33 group B, and 5 group C streptococci. Of 314 samples that yielded\n            <jats:italic>S. pyogenes<\/jats:italic>\n            , 300 were positive on CNA-P (96%) and 264 (86%) were positive on SBA. A significantly greater number of\n            <jats:italic>S. pyogenes<\/jats:italic>\n            isolates from these samples were recovered only on CNA-P (50 of 314) compared with the number of isolates recovered only on SBA (14 of 314). In addition, the degree of positivity, a measure of the total numbers of\n            <jats:italic>S. pyogenes<\/jats:italic>\n            isolates on the plate, was significantly higher on CNA-P than on SBA (2.40 versus 2.07;\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001). Interestingly, CNA-P was also found to enhance the hemolytic activity of streptolysin O, allowing detection of streptolysin S-deficient\n            <jats:italic>S. pyogenes<\/jats:italic>\n            strains which might otherwise go undetected on SBA and other isolation media.\n          <\/jats:p>","DOI":"10.1128\/jcm.38.2.643-650.2000","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:52:44Z","timestamp":1577814764000},"page":"643-650","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":8,"title":["A New Alkaline pH-Adjusted Medium Enhances Detection of \u03b2-Hemolytic Streptococci by Minimizing Bacterial Interference Due to\n            <i>Streptococcus salivarius<\/i>"],"prefix":"10.1128","volume":"38","author":[{"given":"Karen P.","family":"Dierksen","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology, University of Otago, Dunedin, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nancy L.","family":"Ragland","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology, University of Otago, Dunedin, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John R.","family":"Tagg","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Microbiology, University of Otago, Dunedin, New Zealand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(80)90045-5"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.88.2.149"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.97.6.949"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/173.2.365"},{"key":"e_1_3_2_6_2","unstructured":"Breese\nB. 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