{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T09:23:12Z","timestamp":1792660992278,"version":"4.5.0"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2000,2,1]],"date-time":"2000-02-01T00:00:00Z","timestamp":949363200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2000,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We have designed species-specific oligonucleotides which permit the differential detection of two species of cestodes,\n            <jats:italic>Taenia saginata<\/jats:italic>\n            and\n            <jats:italic>Taenia solium<\/jats:italic>\n            . The oligonucleotides contain sequences established for two previously reported, noncoding DNA fragments cloned from a genomic library of\n            <jats:italic>T. saginata<\/jats:italic>\n            . The first, which is\n            <jats:italic>T. saginata<\/jats:italic>\n            specific (fragment HDP1), is a repetitive sequence with a 53-bp monomeric unit repeated 24 times in direct tandem along the 1,272-bp fragment. From this sequence the two oligonucleotides that were selected (oligonucleotides PTs4F1 and PTs4R1) specifically amplified genomic DNA (gDNA) from\n            <jats:italic>T. saginata<\/jats:italic>\n            but not\n            <jats:italic>T. solium<\/jats:italic>\n            or other related cestodes and had a sensitivity down to 10 pg of\n            <jats:italic>T. saginata<\/jats:italic>\n            gDNA. The second DNA fragment (fragment HDP2; 3,954 bp) hybridized to both\n            <jats:italic>T. saginata<\/jats:italic>\n            and\n            <jats:italic>T. solium<\/jats:italic>\n            DNAs and was not a repetitive sequence. Three oligonucleotides (oligonucleotides PTs7S35F1, PTs7S35F2, and PTs7S35R1) designed from the sequence of HDP2 allowed the differential amplification of gDNAs from\n            <jats:italic>T. saginata<\/jats:italic>\n            ,\n            <jats:italic>T. solium<\/jats:italic>\n            , and\n            <jats:italic>Echinococcus granulosus<\/jats:italic>\n            in a multiplex PCR, which exhibits a sensitivity of 10 pg.\n          <\/jats:p>","DOI":"10.1128\/jcm.38.2.737-744.2000","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:52:50Z","timestamp":1577814770000},"page":"737-744","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":80,"title":["Differential Diagnosis of\n            <i>Taenia saginata<\/i>\n            and\n            <i>Taenia solium<\/i>\n            Infection by PCR"],"prefix":"10.1128","volume":"38","author":[{"given":"Luis Miguel","family":"Gonza\u0301lez","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III, Centro Nacional de Microbiologia, 28220 Majadahonda, Madrid, Spain1;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estrella","family":"Montero","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III, Centro Nacional de Microbiologia, 28220 Majadahonda, Madrid, Spain1;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leslie J. S.","family":"Harrison","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->University of Edinburgh, Centre for Tropical Veterinary Medicine, Easter Bush, Roslin, Midlothian, Scotland EH25 9RG2; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R. Michael E.","family":"Parkhouse","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Institute for Animal Health, Pirbright Laboratories, Pirbright, Woking, Surrey, England GU24 0NF3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teresa","family":"Garate","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III, Centro Nacional de Microbiologia, 28220 Majadahonda, Madrid, Spain1;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000060686"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.3513309"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1994.50.1.TM0500010033"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(96)00093-5"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.5.1283-1288.1995"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1006\/expr.1995.1061"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.14.5579"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(91)90302-F"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/12.1Part1.387"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(90)90017-G"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/327415a0"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0169-4758(88)90187-1"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(88)90626-5"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.11.3356"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(91)90004-P"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(91)90042-W"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(94)00197-U"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(96)02747-8"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000078768"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.51.070182.004121"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.17.8213"},{"key":"e_1_3_2_23_2","unstructured":"Lewin\nB.\nGenes VI.\n1997\nOxford University Press Inc.\nNew York N.Y"},{"key":"e_1_3_2_24_2","first-page":"99","article-title":"Factors conditioning detection of Taenia saginata antigens in faeces","volume":"37","author":"Machnicka B.","year":"1996","unstructured":"Machnicka B. 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