{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,31]],"date-time":"2026-10-31T00:25:27Z","timestamp":1793406327126,"version":"4.5.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2000,3,1]],"date-time":"2000-03-01T00:00:00Z","timestamp":951868800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2000,3]]},"abstract":"ABSTRACT<\/jats:title>\n \n A multiplex PCR assay for detection of genes for staphylococcal enterotoxins A to E (\n entA<\/jats:italic>\n ,\n entB<\/jats:italic>\n ,\n entC<\/jats:italic>\n ,\n entD<\/jats:italic>\n , and\n entE<\/jats:italic>\n ), toxic shock syndrome toxin 1 (\n tst<\/jats:italic>\n ), exfoliative toxins A and B (\n etaA<\/jats:italic>\n and\n etaB<\/jats:italic>\n ), and intrinsic methicillin resistance (\n mecA<\/jats:italic>\n ) was developed. Detection of\n femA<\/jats:italic>\n was used as an internal positive control. The multiplex PCR assay combined the primers for\n sea<\/jats:italic>\n to\n see<\/jats:italic>\n and\n femA<\/jats:italic>\n in one set and those for\n eta<\/jats:italic>\n ,\n etb<\/jats:italic>\n ,\n tst<\/jats:italic>\n ,\n mecA<\/jats:italic>\n , and\n femA<\/jats:italic>\n in the other set. Validation of the assay was performed using 176 human isolates of\n Staphylococcus aureus<\/jats:italic>\n . This assay offers a very specific, quick, reliable, and inexpensive alternative to conventional PCR assays used in clinical laboratories to identify various staphylococcal toxin genes.\n <\/jats:p>","DOI":"10.1128\/jcm.38.3.1032-1035.2000","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T12:53:44Z","timestamp":1577796824000},"page":"1032-1035","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":545,"title":["Multiplex PCR for Detection of Genes for\n Staphylococcus aureus<\/i>\n Enterotoxins, Exfoliative Toxins, Toxic Shock Syndrome Toxin 1, and Methicillin Resistance"],"prefix":"10.1128","volume":"38","author":[{"given":"Manisha","family":"Mehrotra","sequence":"first","affiliation":[{"name":"Special Project Unit, Bureau of Microbiology, Canadian Science Centre for Human and Animal Health, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gehua","family":"Wang","sequence":"additional","affiliation":[{"name":"Special Project Unit, Bureau of Microbiology, Canadian Science Centre for Human and Animal Health, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendy M.","family":"Johnson","sequence":"additional","affiliation":[{"name":"Special Project Unit, Bureau of Microbiology, Canadian Science Centre for Human and Animal Health, Winnipeg, Manitoba, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/mcpr.1996.0066"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.171.9.4799-4806.1989"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.9.2548-2553.1998"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00261186"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.170.1.34-41.1988"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)66787-0"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00329830"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1699-0463.1993.tb00165.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.4.781"},{"key":"e_1_3_2_11_2","first-page":"284","article-title":"Toxic shock syndrome: role of the environment, the host and the microorganism","volume":"53","author":"Chance T. D.","year":"1996","unstructured":"Chance T. D. Toxic shock syndrome: role of the environment, the host and the microorganism.Br. J. Biomed. Sci.531996284289","journal-title":"Br. J. Biomed. Sci."},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.170.7.2954-2960.1988"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.7.1768-1772.1994"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-97-3-297"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.158.2.513-516.1984"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.mi.43.100189.002111"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.167.2.726-728.1986"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1995.tb07837.x"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.29.3.426-430.1991"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.166.1.29-33.1986"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.169.9.3904-3909.1987"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.2185544"},{"key":"e_1_3_2_23_2","unstructured":"National Committee for Clinical Laboratory Standards\nDocument M2-A5. Performance standards for antimicrobial disk susceptibility tests\n5th ed.\n1993\nNational Committee for Clinical Laboratory Standards\nVillanova Pa"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.180.5.1675"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(90)90481-6"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/107.3.368"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/167.5.997"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-46-9-773"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.61.4.1438-1443.1995"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.28.3.397"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.11.2864-2867.1995"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0732-8893(94)90047-7"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.38.3.1032-1035.2000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.38.3.1032-1035.2000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,4]],"date-time":"2022-03-04T19:26:28Z","timestamp":1646421988000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.38.3.1032-1035.2000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,3]]},"references-count":31,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2000,3]]}},"alternative-id":["10.1128\/JCM.38.3.1032-1035.2000"],"URL":"https:\/\/doi.org\/10.1128\/jcm.38.3.1032-1035.2000","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,3]]},"assertion":[{"value":"1999-08-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"1999-12-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-03-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}