{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T15:09:46Z","timestamp":1770822586210,"version":"3.50.1"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2000,3,1]],"date-time":"2000-03-01T00:00:00Z","timestamp":951868800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2000,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The feasibility of performing a multiplex assay for the detection of human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV) RNAs and hepatitis B virus (HBV) DNA is demonstrated. This assay is based (i) on the coamplification of a 142-bp fragment from the\n            <jats:italic>gag<\/jats:italic>\n            region of the HIV-1 genome and a 142-bp HIV-1 quantitation standard fragment, a 244-bp fragment from the 5\u2032 noncoding region of the HCV genome, and a 104-bp fragment from the pre-C and C gene regions of the HBV genome, using three sets of specific primers; (ii) on the capacity of these four biotinylated PCR products to hybridize to their specific oligonucleotide probe-coated microspheres; and (iii) on the ability of the flow cytometer to discriminate between distinct fluorescent-microsphere categories. Absence of cross-hybridization between the unrelated oligonucleotide probes and PCR products generated by the multiplex reverse transcription-PCR (RT-PCR) and the highly sensitive detection method allowed us to assess unambiguously the HIV-1 viral load and the infectious status of 35 serologically well-established clinical samples and 20 seronegative blood donor plasma samples tested. The results indicate that multiplex RT-PCR and flow cytometer microsphere-based hybridization assays, when combined, provide a rapid, sensitive, and specific method for the quantitation and detection of the major viral agents of infectious diseases in a single plasma sample.\n          <\/jats:p>","DOI":"10.1128\/jcm.38.3.1066-1071.2000","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:53:53Z","timestamp":1577814833000},"page":"1066-1071","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Simultaneous Detection of Multiplex-Amplified Human Immunodeficiency Virus Type 1 RNA, Hepatitis C Virus RNA, and Hepatitis B Virus DNA Using a Flow Cytometer Microsphere-Based Hybridization Assay"],"prefix":"10.1128","volume":"38","author":[{"given":"J.-P.","family":"Defoort","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Immunoanalysis Department, Immunotech, a Beckman-Coulter Company, 13276 Marseille Cedex 9, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M.","family":"Martin","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Immunoanalysis Department, Immunotech, a Beckman-Coulter Company, 13276 Marseille Cedex 9, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Casano","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Immunoanalysis Department, Immunotech, a Beckman-Coulter Company, 13276 Marseille Cedex 9, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Prato","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Immunoanalysis Department, Immunotech, a Beckman-Coulter Company, 13276 Marseille Cedex 9, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Camilla","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Immunoanalysis Department, Immunotech, a Beckman-Coulter Company, 13276 Marseille Cedex 9, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Fert","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Immunoanalysis Department, Immunotech, a Beckman-Coulter Company, 13276 Marseille Cedex 9, France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0890-8508(90)90026-V"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(85)80771-7"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/42.12.1915"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-1759(94)00252-R"},{"key":"e_1_3_2_6_2","unstructured":"Fulwyler M. 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