{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T23:48:13Z","timestamp":1790812093703,"version":"4.1.0"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2000,3,1]],"date-time":"2000-03-01T00:00:00Z","timestamp":951868800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2000,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A newly developed latex agglutination assay for the detection of genus-specific\n            <jats:italic>Leptospira<\/jats:italic>\n            antibodies in human sera was evaluated. The assay is performed by mixing, on an agglutination card, serum with equal volumes of stabilized antigen-coated, dyed test and control latex beads and is read within 2 min. The latex agglutination test was evaluated with groups of serum samples from patients with leptospirosis and control patients from Hawaii, the Seychelles, Thailand, and The Netherlands. The mean overall sensitivity was 82.3%, and the mean overall specificity was 94.6%. The assay is easy to perform and does not require special skills or equipment. The reagents have a long shelf life, even at tropical temperatures. Together, these factors make the assay suitable for use even at the peripheral level of a health care system as a rapid screening test for leptospirosis.\n          <\/jats:p>","DOI":"10.1128\/jcm.38.3.1272-1275.2000","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:54:26Z","timestamp":1577814866000},"page":"1272-1275","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":78,"title":["Simple Latex Agglutination Assay for Rapid Serodiagnosis of Human Leptospirosis"],"prefix":"10.1128","volume":"38","author":[{"given":"Henk L.","family":"Smits","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Biomedical Research, Royal Tropical Institute, 1105 AZ Amsterdam, The Netherlands1;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menno A. W. G.","family":"van der Hoorn","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biomedical Research, Royal Tropical Institute, 1105 AZ Amsterdam, The Netherlands1;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marga G. A.","family":"Goris","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biomedical Research, Royal Tropical Institute, 1105 AZ Amsterdam, The Netherlands1;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"George C.","family":"Gussenhoven","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biomedical Research, Royal Tropical Institute, 1105 AZ Amsterdam, The Netherlands1;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude","family":"Yersin","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Department of Medicine, Victoria Hospital, Victoria, Seychelles2; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Sasaki","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Epidemiology Branch, Department of Health, State of Hawaii, Honolulu, Hawaii3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wiepko J.","family":"Terpstra","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biomedical Research, Royal Tropical Institute, 1105 AZ Amsterdam, The Netherlands1;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudy A.","family":"Hartskeerl","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Biomedical Research, Royal Tropical Institute, 1105 AZ Amsterdam, The Netherlands1;"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(94)90087-6"},{"key":"e_1_3_2_3_2","first-page":"45","article-title":"Nutrition of Leptospira pomona and growth of 13 other serotypes: fractionation of oleic albumin complex and a medium of bovine albumin and polysorbate 80","volume":"26","author":"Ellinghausen H. C.","year":"1965","unstructured":"Ellinghausen H. C. McCullough W. G. Nutrition of Leptospira pomona and growth of 13 other serotypes: fractionation of oleic albumin complex and a medium of bovine albumin and polysorbate 80.Am. J. Vet. Res.2619654551","journal-title":"Am. J. Vet. Res."},{"key":"e_1_3_2_4_2","unstructured":"Faine\nS.\nGuidelines for the control of leptospirosis.\n1982\nWorld Health Organization\nGeneva Switzerland"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/21.1.1"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.1.92-97.1997"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.94.1.27-31.1967"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1139\/m60-052"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.2105\/AJPH.49.8.1074"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(75)92790-7"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(99)90293-6"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.9.2904-2909.1999"},{"key":"e_1_3_2_13_2","first-page":"336","article-title":"Serodiagnosis of human leptospirosis by enzyme-linked-immunosorbent-assay (ELISA)","volume":"236","author":"Terpstra W. 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Predictive value of a single diagnostic test in unselected populations.N. Engl. J. Med.74196611721173","journal-title":"N. Engl. J. 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