{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T01:09:14Z","timestamp":1782522554609,"version":"3.54.5"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2000,4,1]],"date-time":"2000-04-01T00:00:00Z","timestamp":954547200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2000,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A commercially available enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Q fever (PanBio\n            <jats:italic>Coxiella burnetii<\/jats:italic>\n            immunoglobulin M [IgM] ELISA, QFM-200) was compared to the indirect fluorescent antibody test (IFAT) for\n            <jats:italic>C. burnetii<\/jats:italic>\n            IgM and the complement fixation test (CFT). The ELISA demonstrated 92% agreement with the reference method (IFAT), and gave a sensitivity of 99% (69 of 70 samples) and a specificity of 88% (106 of 121). Specificity can be increased with confirmation by IFAT. CFT was found to have a specificity of 90% (107 of 119), although it was lacking in sensitivity (73%; 51 of 70). No cross-reactivity was observed in the ELISA with serum samples from patients with mycoplasma (\n            <jats:italic>n<\/jats:italic>\n            = 6), chlamydia (\n            <jats:italic>n<\/jats:italic>\n            = 5), or legionella (\n            <jats:italic>n<\/jats:italic>\n            = 4) infections, although 2 of 5 patients with leptospirosis and 1 of 4 samples containing rheumatoid factor (RF) demonstrated positive results in the ELISA. Results indicate that the performance of the PanBio\n            <jats:italic>C. burnetii<\/jats:italic>\n            (Q fever) IgM ELISA (\n            <jats:italic>F<\/jats:italic>\n            = 187) is superior to that of CFT (\n            <jats:italic>F<\/jats:italic>\n            = 163), and consequently the ELISA should be a useful aid in the diagnosis of acute Q fever.\n          <\/jats:p>","DOI":"10.1128\/jcm.38.4.1645-1647.2000","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:55:34Z","timestamp":1577814934000},"page":"1645-1647","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":53,"title":["Comparison of a Commercial Enzyme-Linked Immunosorbent Assay with Immunofluorescence and Complement Fixation Tests for Detection of\n            <i>Coxiella burnetii<\/i>\n            (Q Fever) Immunoglobulin M"],"prefix":"10.1128","volume":"38","author":[{"given":"Peter R.","family":"Field","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead,1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jody L.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->PanBio, Brisbane,2 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avelina","family":"Santiago","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead,1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Dickeson","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead,1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sau-Wan","family":"Chan","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead,1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W. T.","family":"Ho","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead,1"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alan M.","family":"Murphy","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Viral Diagnostic and Referral Laboratory, North Ryde,3 Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea J.","family":"Cuzzubbo","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->PanBio, Brisbane,2 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter L.","family":"Devine","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->PanBio, Brisbane,2 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.9.2451-2455.1992"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.4.3.384-386.1997"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.eurheartj.a062016"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.22.4.484-487.1985"},{"key":"e_1_3_2_6_2","first-page":"499","article-title":"Serological reaction to Legionella pneumophila group 4 in a patient with Q fever","volume":"158","author":"Dwyer D. 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A.\n1991\n285\n310\nCRC Press\nBoca Raton Fla"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.39.2.977-981.1983"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.5694\/j.1326-5377.1970.tb84481.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.3109\/00313028009077101"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.26.10.1978-1982.1988"},{"key":"e_1_3_2_17_2","first-page":"394","article-title":"Evaluation of the complement fixation and indirect immunofluorescence tests in the early diagnosis of primary Q fever","volume":"4","author":"P\u00e9ter O.","year":"1985","unstructured":"P\u00e9ter O. Dupuis G. Burgdorfer W. Peacock M. Evaluation of the complement fixation and indirect immunofluorescence tests in the early diagnosis of primary Q fever.Eur. J. Clin. Microbiol. Infect. Dis.41985394396","journal-title":"Eur. J. Clin. Microbiol. Infect. 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