{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T23:31:32Z","timestamp":1791415892655,"version":"4.3.3"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2000,5,1]],"date-time":"2000-05-01T00:00:00Z","timestamp":957139200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2000,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Since Lyme arthritis was first described in the United States, it has now been reported in many countries of Europe. However, very few strains of the causative bacterium,\n            <jats:italic>Borrelia burgdorferi<\/jats:italic>\n            , have been isolated from synovial samples. For this reason, different molecular direct typing methods were developed recently to assess which species could be involved in Lyme arthritis in Europe. We developed a simple oligonucleotide typing method with PCR fragments from the flagellin gene of\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            sensu lato, which is able to differentiate seven different\n            <jats:italic>Borrelia<\/jats:italic>\n            species. Among 10 consecutive PCR-positive patients with Lyme arthritis from the northeastern France, two species were identified in synovial samples:\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            sensu stricto in 9 cases and\n            <jats:italic>B. garinii<\/jats:italic>\n            in 1 case. Conversely, all\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            sensu lato species detected in 10 consecutive PCR-positive biopsies from a second set of patients with erythema migrans from the same geographical area were identified as either\n            <jats:italic>B. afzelii<\/jats:italic>\n            or\n            <jats:italic>B. garinii<\/jats:italic>\n            (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001). These results indicate that\n            <jats:italic>B. burgdorferi<\/jats:italic>\n            sensu stricto is the principal but not the only\n            <jats:italic>Borrelia<\/jats:italic>\n            species involved in Lyme arthritis in northeastern France.\n          <\/jats:p>","DOI":"10.1128\/jcm.38.5.1895-1900.2000","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:56:17Z","timestamp":1577814977000},"page":"1895-1900","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":82,"title":["Direct Molecular Typing of\n            <i>Borrelia burgdorferi<\/i>\n            Sensu Lato Species in Synovial Samples from Patients with Lyme Arthritis"],"prefix":"10.1128","volume":"38","author":[{"given":"B.","family":"Jaulhac","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Institut de Bacte\u0301riologie de la Faculte\u0301 de Me\u0301decine, Universite\u0301 Louis Pasteur and Ho\u0302pitaux Universitaires de Strasbourg,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Heller","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Institut de Bacte\u0301riologie de la Faculte\u0301 de Me\u0301decine, Universite\u0301 Louis Pasteur and Ho\u0302pitaux Universitaires de Strasbourg,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. 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