{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,11]],"date-time":"2026-04-11T03:44:06Z","timestamp":1775879046371,"version":"3.50.1"},"reference-count":10,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2000,5,1]],"date-time":"2000-05-01T00:00:00Z","timestamp":957139200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2000,5]]},"abstract":"ABSTRACT<\/jats:title>\n \n Cryptococcus neoformans MAT<\/jats:italic>\n \u03b1 and\n MAT<\/jats:italic>\n a pheromones were amplified by direct PCR. Nucleotide sequence analyses revealed unique restriction enzyme sites. Sixty strains were used to devise a restriction fragment length polymorphism typing scheme that yielded three variety-specific patterns. Additionally, pheromone-specific PCR allowed easier identification of diploid\n C. neoformans<\/jats:italic>\n strains than flow cytometry.\n <\/jats:p>","DOI":"10.1128\/jcm.38.5.2007-2009.2000","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:56:40Z","timestamp":1577815000000},"page":"2007-2009","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":70,"title":["Direct PCR of\n Cryptococcus neoformans MAT<\/i>\n \u03b1 and\n MAT<\/i>\n a<\/b>\n Pheromones To Determine Mating Type, Ploidy, and Variety: a Tool for Epidemiological and Molecular Pathogenesis Studies"],"prefix":"10.1128","volume":"38","author":[{"given":"Sudha","family":"Chaturvedi","sequence":"first","affiliation":[{"name":"Mycology Laboratory, Wadsworth Center, New York State Department of Health,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Birgit","family":"Rodeghier","sequence":"additional","affiliation":[{"name":"Mycology Laboratory, Wadsworth Center, New York State Department of Health,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jinjiang","family":"Fan","sequence":"additional","affiliation":[{"name":"Mycology Laboratory, Wadsworth Center, New York State Department of Health,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carol M.","family":"McClelland","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Texas Health Sciences Center at San Antonio, San Antonio, Texas2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brian L.","family":"Wickes","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Texas Health Sciences Center at San Antonio, San Antonio, Texas2"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vishnu","family":"Chaturvedi","sequence":"additional","affiliation":[{"name":"Mycology Laboratory, Wadsworth Center, New York State Department of Health,1 and"},{"name":"Department of Biomedical Sciences, School of Public Health, State University of New York at Albany,3 Albany, New York, and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","unstructured":"Casadevall\nA.\nPerfect\nJ. 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