{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:02:43Z","timestamp":1790208163099,"version":"4.1.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2000,6,1]],"date-time":"2000-06-01T00:00:00Z","timestamp":959817600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2000,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A recent case report of a child infected with enterohemorrhagic<jats:italic>Escherichia coli<\/jats:italic>(EHEC) of serotype O118:H16 in Bavaria, in association with the isolation of a bovine O118 strain on the same farm (A. Weber, H. Klie, H. Richter, P. Gallien, M. Timm, and K. W. Perlberg, Berl. Muench. Tieraerztl. Wochenschr. 110:211\u2013213, 1997), prompted us to investigate the relationship between bovine and human strains of serogroup O118. A total of 29 human O118<jats:italic>E. coli<\/jats:italic>strains from Europe (21), Canada (4), and Peru (4) were compared by virulence typing and macrorestriction analysis with 7 bovine O118 EHEC strains isolated in Bavaria. Twenty-five of the human strains were characterized as EHEC. By serotyping and determination of the virulence-associated factors Shiga toxin (<jats:italic>stx1 stx2 stx2<\/jats:italic>variants), intimin (<jats:italic>eae<\/jats:italic>), and EHEC hemolysin (Hly<jats:sub>EHEC<\/jats:sub>), three distinctive groups of O118 human pathogens were identified. Most of the strains belonged to serotype O118:H16, displaying the virulence traits Stx1, intimin, Hly<jats:sub>EHEC<\/jats:sub>, and EspP\/PssA (group 1). In addition, we identified strains of serotype O118:H12 (Stx2d only; group 2) and of serotype O118:H30 (Stx2 and intimin; group 3). Macrorestriction analysis with<jats:italic>Bln<\/jats:italic>I and<jats:italic>Xba<\/jats:italic>I revealed that all strains with a single O118 serotype profile (O118:H12, O118:H16, and O118:H30) belonged to one clonal cluster, irrespective of their origin. Group 1 strains clustered in the same clonal group as the bovine O118:H16 strains. Moreover, four pairs of strains of different origins and indistinguishable by all other methods applied were identified as group 1 strains. Our data support the direct transmission of an EHEC O118:H16 strain from a calf to a 2-year-old boy in the above-mentioned case report. Since bovine and human O118:H16 strains represent the same clones, they must be considered zoonotic EHEC pathogens. In contrast, EHEC strains of serotypes O118:H12 and O118:H30 have been isolated only from humans, indicating a reservoir for certain human O118 EHEC strains other than bovines.<\/jats:p>","DOI":"10.1128\/jcm.38.6.2162-2169.2000","type":"journal-article","created":{"date-parts":[[2020,3,9]],"date-time":"2020-03-09T19:47:11Z","timestamp":1583783231000},"page":"2162-2169","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Enterohemorrhagic<i>Escherichia coli<\/i>(EHEC) Strains of Serogroup O118 Display Three Distinctive Clonal Groups of EHEC Pathogens"],"prefix":"10.1128","volume":"38","author":[{"given":"Lothar H.","family":"Wieler","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Institut fu\u0308r Mikrobiologie und Tierseuchen, Freie Universita\u0308t Berlin, 10115 Berlin,1"},{"name":"<!--label omitted: 2-->Institut fu\u0308r Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universita\u0308t Giessen, 35392 Giessen,2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara","family":"Busse","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Institut fu\u0308r Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universita\u0308t Giessen, 35392 Giessen,2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hartmut","family":"Steinru\u0308ck","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Robert-Koch-Institut, 13353 Berlin,3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lothar","family":"Beutin","sequence":"additional","affiliation":[{"name":"<!--label omitted: 3-->Robert-Koch-Institut, 13353 Berlin,3"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Albert","family":"Weber","sequence":"additional","affiliation":[{"name":"<!--label omitted: 4-->Landesuntersuchungsamt fu\u0308r das Gesundheitswesen Nordbayern, 90419 Nu\u0308rnberg,4 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helge","family":"Karch","sequence":"additional","affiliation":[{"name":"<!--label omitted: 5-->Institut fu\u0308r Hygiene und Mikrobiologie, Ludwig-Maximilians-Universita\u0308t Wu\u0308rzburg, 97080 Wu\u0308rzburg,5 Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georg","family":"Baljer","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Institut fu\u0308r Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universita\u0308t Giessen, 35392 Giessen,2"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"290","article-title":"Zur aktuellen Bedeutung der enteroh\u00e4morrhagischen Escherichia coli (EHEC) in Deutschland (1994\u20131995)","volume":"39","author":"Bockem\u00fchl J.","year":"1996","unstructured":"Bockem\u00fchl J. 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