{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T21:08:05Z","timestamp":1786568885531,"version":"build-2736575974"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2000,6,1]],"date-time":"2000-06-01T00:00:00Z","timestamp":959817600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2000,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Using broad-spectrum primers, we have amplified, cloned, and sequenced a 620-bp fragment of the \u201c\n            <jats:italic>Tropheryma whippelii<\/jats:italic>\n            \u201d heat shock protein 65 gene (\n            <jats:italic>hsp65<\/jats:italic>\n            ) from the heart valve of a patient with Whipple's endocarditis. The deduced amino acid sequence shows high similarity to those from actinobacteria, confirming that \u201c\n            <jats:italic>T. whippelii<\/jats:italic>\n            \u201d is indeed a member of this phylum. Based on the nucleotide sequence, we have developed a \u201c\n            <jats:italic>T. whippelii<\/jats:italic>\n            \u201d-specific seminested PCR. Seventeen patients shown to be positive by 16S ribosomal DNA (rDNA) PCR and\/or internal transcribed spacer (ITS) PCR were also positive by\n            <jats:italic>hsp65<\/jats:italic>\n            PCR. All 33 control specimens from patients without Whipple's disease and negative for \u201c\n            <jats:italic>T. whippelii<\/jats:italic>\n            \u201d by both seminested 16S rDNA and ITS PCR remained negative. All amplicons digested with\n            <jats:italic>Xho<\/jats:italic>\n            I revealed an identical restriction pattern. Eight of the 17\n            <jats:italic>hsp65<\/jats:italic>\n            amplicons representing all three previously described ITS types were sequenced. Three of the amplicons showed slight differences, but none of the mutations detected affected the amino acid sequence of the corresponding protein. We conclude that the\n            <jats:italic>hsp65<\/jats:italic>\n            gene is a suitable target for the specific detection of \u201c\n            <jats:italic>T. whippelii<\/jats:italic>\n            .\u201d Its product represents a putative antigen for a future serodiagnostic assay.\n          <\/jats:p>","DOI":"10.1128\/jcm.38.6.2248-2253.2000","type":"journal-article","created":{"date-parts":[[2020,3,9]],"date-time":"2020-03-09T19:47:19Z","timestamp":1583783239000},"page":"2248-2253","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":41,"title":["Cloning and Sequencing of a Part of the Heat Shock Protein 65 Gene (\n            <i>hsp65<\/i>\n            ) of \u201c\n            <i>Tropheryma whippelii<\/i>\n            \u201d and Its Use for Detection of \u201c\n            <i>T. whippelii<\/i>\n            \u201d in Clinical Specimens by PCR"],"prefix":"10.1128","volume":"38","author":[{"given":"Silvia","family":"Morgenegg","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology, University of Zu\u0308rich, 8028 Zu\u0308rich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabrizio","family":"Dutly","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology, University of Zu\u0308rich, 8028 Zu\u0308rich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin","family":"Altwegg","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Department of Medical Microbiology, University of Zu\u0308rich, 8028 Zu\u0308rich, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"1495","article-title":"Spondylodiscitis caused by Tropheryma whippelii","volume":"126","author":"Altwegg M.","year":"1996","unstructured":"Altwegg M. Fleisch-Marx A. Goldenberger D. Hailemariam S. Schaffner A. Kissling R. Spondylodiscitis caused by Tropheryma whippelii.Schweiz. Med. Wochenschr.126199614951499","journal-title":"Schweiz. Med. Wochenschr."},{"key":"e_1_3_2_3_2","first-page":"399","article-title":"Relapsing Whipple's disease presenting with hypopituitarism","volume":"50","author":"Br\u00e4ndle M.","year":"1999","unstructured":"Br\u00e4ndle M. Ammann P. Spinas G. A. Dutly F. Galeazzi R. L. Schmid C. Altwegg M. Relapsing Whipple's disease presenting with hypopituitarism.Clin. Endocrinol.501999399403","journal-title":"Clin. Endocrinol."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.59.1.79-90.1991"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-46-4-340"},{"key":"e_1_3_2_6_2","unstructured":"Dobbins\nW. O.\nIII.\nWhipple's disease.\n1987\nCharles C Thomas\nSpringfield Ill"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199502093320611"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)01776-6"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.4.818-823.1996"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.11.2733-2739.1997"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-131-2-199907200-00007"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1995.tb02216.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-49-4-1701"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.2.595-599.2000"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-46-4-1078"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200003023420903"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199207303270501"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.4.964-970.1999"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199502093320604"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.1.139-147.1998"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1086\/514089"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.4.817-822.1997"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)03065-2"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.2.175-178.1993"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1086\/314669"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(91)90545-Z"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.1.19"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.38.6.2248-2253.2000","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.38.6.2248-2253.2000","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T00:39:54Z","timestamp":1646440794000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.38.6.2248-2253.2000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,6]]},"references-count":26,"aliases":["10.1128\/.38.6.2248-2253.2000"],"journal-issue":{"issue":"6","published-print":{"date-parts":[[2000,6]]}},"alternative-id":["10.1128\/JCM.38.6.2248-2253.2000"],"URL":"https:\/\/doi.org\/10.1128\/jcm.38.6.2248-2253.2000","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2000,6]]},"assertion":[{"value":"1999-12-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-03-24","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}