{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T01:38:08Z","timestamp":1792719488198,"version":"4.5.0"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2000,6,1]],"date-time":"2000-06-01T00:00:00Z","timestamp":959817600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2000,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Saliva from subjects with amebic liver abscess (ALA), acute amebic colitis, asymptomatic infection with\n            <jats:italic>Entamoeba histolytica<\/jats:italic>\n            or\n            <jats:italic>Entamoeba dispar<\/jats:italic>\n            , and uninfected controls was tested by enzyme-linked immunosorbent assay (ELISA) for the presence of\n            <jats:italic>E. histolytica<\/jats:italic>\n            galactose-inhibitable lectin antigen and salivary immunoglobulin (IgG) antibodies to a recombinant cysteine-rich lectin-derived protein (LC3). Salivary lectin antigen was found in 65.8% of subjects with acute colitis, compared to 22.2% of those convalescent from ALA, 10.0% with asymptomatic\n            <jats:italic>E. histolytica<\/jats:italic>\n            infection, 9.8% with\n            <jats:italic>E. dispar<\/jats:italic>\n            infection, and 2.6% of controls (subjects from the United States and study patients with nonamebic diarrhea) (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001 for each compared to values for subjects with colitis). Salivary anti-LC3 IgG antibodies were found in 92% of ALA patients regardless of duration of illness and in 83.3% of colitis patients who were symptomatic for at least 7 days (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001 compared to other study groups). Serum anti-LC3 IgG antibodies were detected in 56.3% of subjects with acute colitis, 100% of subjects with ALA or prolonged colitis, 45% of subjects with asymptomatic\n            <jats:italic>E. histolytica<\/jats:italic>\n            infection, 32.3% of subjects with\n            <jats:italic>E. dispar<\/jats:italic>\n            infection, and 23.4% of diarrhea controls. In comparison to ELISA for serum anti-LC3 IgG antibodies, the salivary lectin antigen assay is a more sensitive and specific test for acute amebic colitis. Detection of salivary anti-LC3 IgG antibodies by ELISA is an effective means for the diagnosis of ALA and prolonged cases of amebic colitis.\n          <\/jats:p>","DOI":"10.1128\/jcm.38.6.2344-2347.2000","type":"journal-article","created":{"date-parts":[[2020,3,9]],"date-time":"2020-03-09T19:48:37Z","timestamp":1583783317000},"page":"2344-2347","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":38,"title":["Diagnosis of Invasive Amebiasis by Enzyme-Linked Immunosorbent Assay of Saliva To Detect Amebic Lectin Antigen and Anti-Lectin Immunoglobulin G Antibodies"],"prefix":"10.1128","volume":"38","author":[{"given":"Mohamed D.","family":"Abd-Alla","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->El-Hussein University Hospital, Cairo, Egypt1;"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Terry F. H. G.","family":"Jackson","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Medical Research Council (Nat)(al)2 and"},{"name":"<!--label omitted: 3-->University of Natal School of Medicine,3 Durban, South Africa; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Selvan","family":"Reddy","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Medical Research Council (Nat)(al)2 and"},{"name":"<!--label omitted: 3-->University of Natal School of Medicine,3 Durban, South Africa; and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan I.","family":"Ravdin","sequence":"additional","affiliation":[{"name":"<!--label omitted: 4-->Department of Medicine, University of Minnesota, Minneapolis, Minnesota4"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1992.47.800"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.11.2845-2850.1993"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1998.59.431"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/174.1.157"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/164.3.613"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1993.tb04926.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0074-02761995000400016"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.10.2558-2561.1995"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1994.51.454"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1997.tb00456.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.12.3358-3360.1997"},{"key":"e_1_3_2_13_2","first-page":"585","article-title":"Saliva: clinical and diagnostic usefulness. 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