{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T16:12:01Z","timestamp":1793203921993,"version":"4.5.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2000,7,1]],"date-time":"2000-07-01T00:00:00Z","timestamp":962409600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2000,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A whole-cell hybridization assay with fluorescent oligonucleotide probes derived from the 16S rRNA sequence of\n            <jats:italic>Brucella abortus<\/jats:italic>\n            in combination with flow cytometry has been developed. With the three fluorescent probes selected, a positive signal was observed with all the representative strains of the species and biovars of\n            <jats:italic>Brucella<\/jats:italic>\n            and with a total of nine different\n            <jats:italic>Brucella<\/jats:italic>\n            clinical isolates. Using the B9 probe in the hybridization assay, it was possible to discriminate between\n            <jats:italic>Brucella suis<\/jats:italic>\n            biovars 2, 3, 4, and 5 and almost all the other\n            <jats:italic>Brucella<\/jats:italic>\n            spp. On the basis of differences in fluorescence intensities, no discrimination was established between\n            <jats:italic>Brucella<\/jats:italic>\n            spp. and other phylogenetically related microorganisms. No positive fluorescence signals were detected with any of the bacteria showing serological cross-reactions with\n            <jats:italic>Brucella<\/jats:italic>\n            spp. and with a total of 17 clinical isolates not belonging to the genus\n            <jats:italic>Brucella<\/jats:italic>\n            . These results suggest that the 16S rRNA whole-cell hybridization technique could be a valuable diagnostic tool for the detection and identification of\n            <jats:italic>Brucella<\/jats:italic>\n            spp.\n          <\/jats:p>","DOI":"10.1128\/jcm.38.7.2768-2771.2000","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:57:23Z","timestamp":1577815043000},"page":"2768-2771","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":6,"title":["Fluorescent Whole-Cell Hybridization with 16S rRNA-Targeted Oligonucleotide Probes To Identify\n            <i>Brucella<\/i>\n            spp. by Flow Cytometry"],"prefix":"10.1128","volume":"38","author":[{"given":"Luis","family":"Ferna\u0301ndez-Lago","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Microbiolog\u0131\u0301a y Gene\u0301tica, Edificio Departamental,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. Javier","family":"Vallejo","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Microbiolog\u0131\u0301a y Gene\u0301tica, Edificio Departamental,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ignacio","family":"Trujillano","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Departamento de Medicina Preventiva y Microbiolog\u0131\u0301a Me\u0301dica, Facultad de Medicina,2 Universidad de Salamanca, 37007 Salamanca, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nieves","family":"Vizcai\u0301no","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Departamento de Microbiolog\u0131\u0301a y Gene\u0301tica, Edificio Departamental,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/18.6.914"},{"key":"e_1_3_2_3_2","unstructured":"Alton\nG. G.\nJones\nL. 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