{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T01:04:47Z","timestamp":1785632687671,"version":"3.56.0"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000]]},"abstract":"<jats:p>A noninvasive diagnostic test for amebic liver abscess is needed, because amebic and bacterial abscesses appear identical on ultrasound or computer tomography and because it is rarely possible to identify<jats:italic>Entamoeba histolytica<\/jats:italic> in stool specimens from patients with amebic liver abscess. Here we report a method of detection in serum of circulating <jats:italic>E. histolytica<\/jats:italic> Gal\/GalNAc lectin to diagnose amebic liver abscess, which was used in patients from Dhaka, Bangladesh. The TechLab <jats:italic>E. histolytica<\/jats:italic> II test (which differentiates the true pathogen <jats:italic>E. histolytica<\/jats:italic> from<jats:italic>Entamoeba dispar<\/jats:italic>) detected Gal\/GalNAc lectin in the sera of 22 of 23 (96%) amebic liver abscess patients tested prior to treatment with the antiamebic drug metronidazole and 0 of 70 (0%) controls. After 1 week of treatment with metronidazole, 9 of 11 (82%) patients became serum lectin antigen negative. The sensitivity of the <jats:italic>E. histolytica<\/jats:italic> II antigen detection test for intestinal infection was also evaluated. Antigen detection identified <jats:italic>E. histolytica<\/jats:italic> infection in 50 samples from 1,164 asymptomatic preschool children aged 2 to 5 years, including 16 of 16 (100%) culture-positive specimens. PCR analysis of stool specimens was used to confirm that most antigen-positive but culture-negative specimens were true-positive: PCR identified parasite DNA in 27 of 34 (79%) of the antigen-positive, culture-negative stool specimens. Antigen detection was a more sensitive test for infection than antilectin antibodies, which were detected in only 76 of 98 (78%) amebic liver abscess patients and in 26 of 50 (52%) patients with intestinal infection. We conclude that the TechLab <jats:italic>E. histolytica<\/jats:italic> II kit is a sensitive means to diagnose hepatic and intestinal amebiasis prior to the institution of metronidazole treatment.<\/jats:p>","DOI":"10.1128\/jcm.38.9.3235-3239.2000","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:58:44Z","timestamp":1577815124000},"page":"3235-3239","source":"Crossref","is-referenced-by-count":181,"title":["Diagnosis of Amebic Liver Abscess and Intestinal Infection with the TechLab Entamoeba histolytica II Antigen Detection and Antibody Tests"],"prefix":"10.1128","volume":"38","author":[{"given":"Rashidul","family":"Haque","sequence":"first","affiliation":[{"name":"Centre for Health and Population Research, ICDDR,B,\n1\n and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nasir Uddin","family":"Mollah","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hepatology, Bangabandhu Sheikh Mujib Medical University,\n2\n Dhaka, Bangladesh, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ibne Karim M.","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hepatology, Bangabandhu Sheikh Mujib Medical University,\n2\n Dhaka, Bangladesh, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khorshed","family":"Alam","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Hepatology, Bangabandhu Sheikh Mujib Medical University,\n2\n Dhaka, Bangladesh, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleida","family":"Eubanks","sequence":"additional","affiliation":[{"name":"TechLab, Inc., Blacksburg,\n3\n and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Lyerly","sequence":"additional","affiliation":[{"name":"TechLab, Inc., Blacksburg,\n3\n and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr.","given":"William A.","family":"Petri","sequence":"additional","affiliation":[{"name":"Departments of Medicine, Microbiology and Pathology, University of Virginia, Charlottesville,\n4\n Virginia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T18:42:34Z","timestamp":1627584154000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.38.9.3235-3239.2000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000]]},"references-count":0,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2000]]}},"URL":"https:\/\/doi.org\/10.1128\/jcm.38.9.3235-3239.2000","relation":{},"ISSN":["1098-660X","0095-1137"],"issn-type":[{"value":"1098-660X","type":"electronic"},{"value":"0095-1137","type":"print"}],"subject":[],"published":{"date-parts":[[2000]]}}}