{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T02:14:47Z","timestamp":1792721687805,"version":"4.5.0"},"reference-count":0,"publisher":"American Society for Microbiology","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2000]]},"abstract":"<jats:p>We have analyzed possible qualitative and quantitative differences in antigen expression between <jats:italic>Helicobacter pylori<\/jats:italic> strains isolated from the antrum and different locations in the duodenum of 21 duodenal ulcer (DU) patients and 20 asymptomatic subjects (AS) by enzyme-linked immunosorbent assay (ELISA) and inhibition ELISA. Almost all antral and duodenal strains grown in vitro expressed the<jats:italic>N<\/jats:italic>-acetyl-neuroaminyllactose-binding hemagglutinin, flagellins (subunits FlaA and FlaB), urease, a 26-kDa protein, and a neutrophil-activating protein. In 75% of both the DU patients and the AS, antral <jats:italic>H. pylori<\/jats:italic> strains expressed either the blood group antigen Lewis y (Le<jats:sup>y<\/jats:sup>) alone or together with the Le<jats:sup>x<\/jats:sup> antigen. However, duodenal <jats:italic>H. pylori<\/jats:italic>strains of DU patients expressed Le<jats:sup>y<\/jats:sup> antigen more frequently than corresponding strains of AS (<jats:italic>P<\/jats:italic> &lt; 0.05). Presence of Le<jats:sup>y<\/jats:sup> on <jats:italic>H. pylori<\/jats:italic> was related to the degree of active duodenitis (<jats:italic>P<\/jats:italic> &lt; 0.05). Duodenal <jats:italic>H. pylori<\/jats:italic> strains isolated from AS were significantly more often Lewis nontypeable than duodenal strains of DU patients (<jats:italic>P<\/jats:italic> &lt; 0.01). Presence of <jats:italic>H. pylori<\/jats:italic> blood group antigen-binding adhesin (BabA) was significantly higher on both antral and duodenal strains isolated from DU patients than on corresponding strains isolated from AS (<jats:italic>P<\/jats:italic> &lt; 0.05). BabA-positive duodenal <jats:italic>H. pylori<\/jats:italic> strains isolated from DU patients were associated with active duodenitis more frequently than corresponding strains isolated from AS (<jats:italic>P<\/jats:italic> &lt; 0.01). Infection with <jats:italic>H. pylori<\/jats:italic> strains positive for Le<jats:sup>y<\/jats:sup> and BabA in the duodenum is associated with development of duodenal ulcer formation.<\/jats:p>","DOI":"10.1128\/jcm.38.9.3436-3441.2000","type":"journal-article","created":{"date-parts":[[2019,12,31]],"date-time":"2019-12-31T17:59:14Z","timestamp":1577815154000},"page":"3436-3441","source":"Crossref","is-referenced-by-count":34,"title":["Differences in Surface-Exposed Antigen Expression between Helicobacter pylori Strains Isolated from Duodenal Ulcer Patients and from Asymptomatic Subjects"],"prefix":"10.1128","volume":"38","author":[{"given":"Ann-Catrin E.","family":"Thoreson","sequence":"first","affiliation":[{"name":"Departments of Medical Microbiology and Immunology\n1\n and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annika","family":"Hamlet","sequence":"additional","affiliation":[{"name":"Departments of Medical Microbiology and Immunology\n1\n and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janet","family":"C\u0327elik","sequence":"additional","affiliation":[{"name":"Microbiology and Tumor-Biology Center, Karolinska Institute, S-171 77 Stockholm,\n2\nand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mona","family":"Bystro\u0308m","sequence":"additional","affiliation":[{"name":"AstraZeneca, Ume\u00e5,\n3\n Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susanne","family":"Nystro\u0308m","sequence":"additional","affiliation":[{"name":"AstraZeneca, Ume\u00e5,\n3\n Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"Olbe","sequence":"additional","affiliation":[{"name":"Surgery,\n4\n Go\u0308teborg University, S-413 46 Go\u0308teborg,"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ann-Mari","family":"Svennerholm","sequence":"additional","affiliation":[{"name":"Departments of Medical Microbiology and Immunology\n1\n and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","deposited":{"date-parts":[[2021,7,29]],"date-time":"2021-07-29T18:42:54Z","timestamp":1627584174000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.38.9.3436-3441.2000"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000]]},"references-count":0,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2000]]}},"URL":"https:\/\/doi.org\/10.1128\/jcm.38.9.3436-3441.2000","relation":{},"ISSN":["1098-660X","0095-1137"],"issn-type":[{"value":"1098-660X","type":"electronic"},{"value":"0095-1137","type":"print"}],"subject":[],"published":{"date-parts":[[2000]]}}}