{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T22:50:20Z","timestamp":1779144620114,"version":"3.51.4"},"reference-count":10,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2001,1,1]],"date-time":"2001-01-01T00:00:00Z","timestamp":978307200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2001,1]]},"abstract":"ABSTRACT<\/jats:title>\n \n A method was developed for extracting cryptosporidial DNA from stained fecal smears on glass microscope slides. The correct genotype of\n Cryptosporidium parvum<\/jats:italic>\n was amplified by PCR from 89 (85%) of 105 smears following conventional staining but not from negative controls. This technique may have applications for analysis of other infectious agents.\n <\/jats:p>","DOI":"10.1128\/jcm.39.1.401-403.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:03:40Z","timestamp":1027764220000},"page":"401-403","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":28,"title":["Extraction and Genotyping of\n Cryptosporidium parvum<\/i>\n DNA from Fecal Smears on Glass Slides Stained Conventionally for Direct Microscope Examination"],"prefix":"10.1128","volume":"39","author":[{"given":"C.","family":"Amar","sequence":"first","affiliation":[{"name":"Food Safety Microbiology Laboratory, PHLS Division of Gastrointestinal Infections, Central Public Health Laboratory, London, NW9 5HT,1 and"},{"name":"School of Chemical and Life Sciences, University of Greenwich, Woolwich, London, SE18 8PF,2 United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Pedraza-D\u0131\u0301az","sequence":"additional","affiliation":[{"name":"Food Safety Microbiology Laboratory, PHLS Division of Gastrointestinal Infections, Central Public Health Laboratory, London, NW9 5HT,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"McLauchlin","sequence":"additional","affiliation":[{"name":"Food Safety Microbiology Laboratory, PHLS Division of Gastrointestinal Infections, Central Public Health Laboratory, London, NW9 5HT,1 and"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Anonymous\nPHLS Standard Operating Procedure B.SOP 30. Investigation of faeces specimens.\n1999\nPublic Health Laboratory Service\nLondon United Kingdom"},{"key":"e_1_3_2_3_2","unstructured":"Arrowood\nM. J.\nDiagnosis\nCryptosporidium and cryptosporidiosis.\nFayer\nR.\n1997\n43\n64\nCRC Press Inc.\nBoca Raton Fla"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.3.495-503.1990"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.38.12.1337"},{"key":"e_1_3_2_6_2","doi-asserted-by":"crossref","unstructured":"Fayer\nR.\nSpeer\nC. A.\nDubey\nJ. P.\nThe general biology of Cryptosporidium Cryptosporidium and cryptosporidiosis.\nFayer\nR.\n1997\n1\n41\nCRC Press Inc.\nBoca Raton Fla","DOI":"10.1201\/9781351071260-1"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.10.3153-3158.1999"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(99)00109-5"},{"key":"e_1_3_2_9_2","unstructured":"Pedraza-D\u0131\u0301az S. C. Amar G. L. Nichols and J. McLauchlin. 2000. The development of a nested PCR procedure for amplification of the Cryptosporidium oocyst wall protein (COWP) gene and analysis of 2128 cryptosporidiosis cases. Emerg. Infect. Dis. in press."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1097(97)00115-8"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0404.980424"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.39.1.401-403.2001","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.39.1.401-403.2001","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T01:01:12Z","timestamp":1646442072000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.39.1.401-403.2001"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,1]]},"references-count":10,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2001,1]]}},"alternative-id":["10.1128\/JCM.39.1.401-403.2001"],"URL":"https:\/\/doi.org\/10.1128\/jcm.39.1.401-403.2001","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,1]]},"assertion":[{"value":"2000-08-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2000-10-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}