{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T19:23:50Z","timestamp":1792265030493,"version":"4.4.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2001,2,1]],"date-time":"2001-02-01T00:00:00Z","timestamp":980985600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2001,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Identification of\n                    <jats:italic>Bartonella<\/jats:italic>\n                    species is of increasing importance as the number of infections in which these bacteria are involved increases. To date, these gram-negative bacilli have been identified by various serological, biochemical, and genotypic methods. However, the development of alternative tools is required, principally to circumvent a major risk of contamination during sample manipulation. The aim of our study was to investigate the possible identification of various\n                    <jats:italic>Bartonella<\/jats:italic>\n                    species by comparison of RNA polymerase beta-subunit gene (\n                    <jats:italic>rpoB<\/jats:italic>\n                    ) sequences. This approach has previously been shown to be useful for the identification of members of the family\n                    <jats:italic>Enterobacteriaceae<\/jats:italic>\n                    (C. M. Mollet, M. Drancourt, and D. Raoult, Mol. Microbiol. 26:1005\u20131011, 1997). Following PCR amplification with specific oligonucleotides, a 825-bp region of the\n                    <jats:italic>rpoB<\/jats:italic>\n                    gene was sequenced from 13 distinct\n                    <jats:italic>Bartonella<\/jats:italic>\n                    strains. Analysis of these sequences allowed selection of three restriction enzymes (\n                    <jats:italic>Apo<\/jats:italic>\n                    I,\n                    <jats:italic>Alu<\/jats:italic>\n                    I, and\n                    <jats:italic>Afl<\/jats:italic>\n                    III) useful for discerning the different strains by PCR-restriction fragment length polymorphism (PCR-RFLP) analysis. To confirm the potential value of such an approach for identification of\n                    <jats:italic>Bartonella<\/jats:italic>\n                    , the\n                    <jats:italic>rpoB<\/jats:italic>\n                    PCR was then applied to 94 clinical samples, and the results obtained were identical to those obtained by our reference PCR method. Twenty-four isolates were also adequately identified by PCR-RFLP analysis. In all cases, our results were in accordance with those of the reference method. Moreover, conserved regions of DNA were chosen as suitable primer targets for PCR amplification of a 439-bp fragment which can be easily sequenced.\n                  <\/jats:p>","DOI":"10.1128\/jcm.39.2.430-437.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T06:06:23Z","timestamp":1027749983000},"page":"430-437","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":233,"title":["Use of\n                    <i>rpoB<\/i>\n                    Gene Analysis for Detection and Identification of\n                    <i>Bartonella<\/i>\n                    Species"],"prefix":"10.1128","volume":"39","author":[{"given":"Patricia","family":"Renesto","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->Unite\u0301 des Rickettsies, CNRS UPRES-A 6020, Faculte\u0301 de Me\u0301decine, Universite\u0301 de la Me\u0301diterrane\u0301e,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanny","family":"Gouvernet","sequence":"additional","affiliation":[{"name":"<!--label omitted: 2-->Service de l'Information Me\u0301dicale, Ho\u0302pital de la Timone,2 13385 Marseille, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Drancourt","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Unite\u0301 des Rickettsies, CNRS UPRES-A 6020, Faculte\u0301 de Me\u0301decine, Universite\u0301 de la Me\u0301diterrane\u0301e,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Veronique","family":"Roux","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Unite\u0301 des Rickettsies, CNRS UPRES-A 6020, Faculte\u0301 de Me\u0301decine, Universite\u0301 de la Me\u0301diterrane\u0301e,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Raoult","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->Unite\u0301 des Rickettsies, CNRS UPRES-A 6020, Faculte\u0301 de Me\u0301decine, Universite\u0301 de la Me\u0301diterrane\u0301e,1 and"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.2.254-260.1996"},{"key":"e_1_3_2_3_2","first-page":"261","article-title":"Differentiation of Bartonella species using restriction endonuclease analysis of PCR-amplified 16S rRNA genes","volume":"129","author":"Birtles R. 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