{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T02:48:43Z","timestamp":1778554123245,"version":"3.51.4"},"reference-count":34,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2001,2,1]],"date-time":"2001-02-01T00:00:00Z","timestamp":980985600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2001,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A reverse line blot (RLB) assay was developed to identify different\n            <jats:italic>Trichinella<\/jats:italic>\n            genotypes. The RLB assay accomplishes detection and specific identification of the different\n            <jats:italic>Trichinella<\/jats:italic>\n            genotypes and relies on hybridization of the amplified 5S ribosomal DNA intergenic spacer regions to specific, membrane-bound oligonucleotide probes. After one single amplification, we were able to detect and genetically identify six sibling species, i.e.,\n            <jats:italic>T. spiralis<\/jats:italic>\n            ,\n            <jats:italic>T. britovi<\/jats:italic>\n            ,\n            <jats:italic>T. nativa<\/jats:italic>\n            ,\n            <jats:italic>T. murrelli<\/jats:italic>\n            ,\n            <jats:italic>T. nelsoni<\/jats:italic>\n            , and\n            <jats:italic>T. pseudospiralis<\/jats:italic>\n            , and two additional\n            <jats:italic>Trichinella<\/jats:italic>\n            genotypes, T6 and T8. Twenty-four\n            <jats:italic>Trichinella<\/jats:italic>\n            strains of different genotypes were unequivocally identified evaluated using one simple PCR-based assay based on single larvae. This assay allows the specific identification of\n            <jats:italic>Trichinella<\/jats:italic>\n            species without the need to passage larvae in laboratory animals.\n          <\/jats:p>","DOI":"10.1128\/jcm.39.2.642-646.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:03:40Z","timestamp":1027764220000},"page":"642-646","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":42,"title":["Detection and Identification of Eight\n            <i>Trichinella<\/i>\n            Genotypes by Reverse Line Blot Hybridization"],"prefix":"10.1128","volume":"39","author":[{"given":"Y. 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