{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T17:08:01Z","timestamp":1779901681797,"version":"3.53.1"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2001,2,1]],"date-time":"2001-02-01T00:00:00Z","timestamp":980985600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2001,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The gene encoding the entire\n            <jats:italic>Babesia equi<\/jats:italic>\n            merozoite antigen 1 (EMA-1) was inserted into a baculovirus transfer vector, and a recombinant virus expressing EMA-1 was isolated. The expressed EMA-1 was transported to the surface of infected insect cells, as judged by an indirect fluorescent-antibody test (IFAT). The expressed EMA-1 was also secreted into the supernatant of a cell culture infected with recombinant baculovirus. Both intracellular and extracellular EMA-1 reacted with a specific antibody in Western blots. The expressed EMA-1 had an apparent molecular mass of 34 kDa that was identical to that of native EMA-1. The secreted EMA-1 was used as an antigen in an enzyme-linked immunosorbent assay (ELISA). The ELISA differentiated\n            <jats:italic>B. equi<\/jats:italic>\n            -infected horse sera from\n            <jats:italic>Babesia caballi<\/jats:italic>\n            -infected horse sera or normal horse sera. The ELISA was more sensitive than the complement fixation test and IFAT. These results demonstrated that the recombinant EMA-1 expressed in insect cells might be a useful diagnostic reagent for detection of antibodies to\n            <jats:italic>B. equi<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jcm.39.2.705-709.2001","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:03:40Z","timestamp":1027764220000},"page":"705-709","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":47,"title":["Expression of\n            <i>Babesia equi<\/i>\n            Merozoite Antigen 1 in Insect Cells by Recombinant Baculovirus and Evaluation of Its Diagnostic Potential in an Enzyme-Linked Immunosorbent Assay"],"prefix":"10.1128","volume":"39","author":[{"given":"Xuenan","family":"Xuan","sequence":"first","affiliation":[{"name":"<!--label omitted: 1-->National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandra","family":"Larsen","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromi","family":"Ikadai","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuya","family":"Tanaka","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ikuo","family":"Igarashi","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideyuki","family":"Nagasawa","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kozo","family":"Fujisaki","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutaka","family":"Toyoda","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoyoshi","family":"Suzuki","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takeshi","family":"Mikami","sequence":"additional","affiliation":[{"name":"<!--label omitted: 1-->National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0264-410X(97)88331-4"},{"key":"e_1_3_2_3_2","first-page":"141","article-title":"Prevalence of equine piroplasmosis in central Mongolia","volume":"64","author":"Avarazed A.","year":"1997","unstructured":"Avarazed A. 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