{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T03:53:43Z","timestamp":1777348423743,"version":"3.51.4"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2002,2,1]],"date-time":"2002-02-01T00:00:00Z","timestamp":1012521600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The antigenic potential of decorin binding protein A\n(DbpA) was evaluated in serodiagnosis of human Lyme borreliosis (LB). The\n            <jats:italic>dbpA<\/jats:italic>\n            was cloned and sequenced from the three pathogenic\n            <jats:italic>Borrelia<\/jats:italic>\n            species common in Europe. Sequence analysis revealed high\ninterspecies heterogeneity. The identity of the predicted amino acid\nsequences was 43 to 62% among\n            <jats:italic>Borrelia burgdorferi<\/jats:italic>\n            sensu stricto,\n            <jats:italic>B. afzelii<\/jats:italic>\n            , and\n            <jats:italic>B. garinii<\/jats:italic>\n            . The respective recombinant\nDbpAs (rDbpAs) were produced and tested as antigens by Western blotting and\nenzyme-linked immunosorbent assay (ELISA). One hundred percent of patients\nwith neuroborreliosis (NB) and 93% of patients with Lyme arthritis (LA)\nreacted positively. Sera from the majority of patients reacted with one\nrDbpA only and had no or low cross-reactivity to other two variant\nproteins. In patients with culture-positive erythema migrans (EM), the\nsensitivity of rDbpA immunoglobulin G (IgG) or IgM ELISA was low. The DbpA\nseems to be a sensitive and specific antigen for the serodiagnosis of LA or\nNB, but not of EM, provided that variants from all three pathogenic\nborrelial species are included in the combined set of\nantigens.\n          <\/jats:p>","DOI":"10.1128\/jcm.40.02.453-460.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:53Z","timestamp":1027763753000},"page":"453-460","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":46,"title":["Species-SpecificSerodiagnosis of Lyme Arthritis and Neuroborreliosis Due to\n            <i>Borreliaburgdorferi<\/i>\n            Sensu Stricto,\n            <i>B. afzelii<\/i>\n            , and\n            <i>B.garinii<\/i>\n            by Using Decorin Binding ProteinA"],"prefix":"10.1128","volume":"40","author":[{"given":"Tero","family":"Heikkila\u0308","sequence":"first","affiliation":[{"name":"Hospital forChildren and Adolescents"},{"name":"Department ofBacteriology and Immunology, Haartman Institute, University ofHelsinki"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ilkka","family":"Seppa\u0308la\u0308","sequence":"additional","affiliation":[{"name":"Department ofBacteriology and Immunology, Haartman Institute, University ofHelsinki"},{"name":"HUCH-Laboratory Diagnostics,Helsinki University Central Hospital, Helsinki"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harri","family":"Saxen","sequence":"additional","affiliation":[{"name":"Hospital forChildren and Adolescents"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jaana","family":"Panelius","sequence":"additional","affiliation":[{"name":"Department ofBacteriology and Immunology, Haartman Institute, University ofHelsinki"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Heta","family":"Yrja\u0308na\u0308inen","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, University ofTurku, Turku, Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pekka","family":"Lahdenne","sequence":"additional","affiliation":[{"name":"Hospital forChildren and Adolescents"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-42-3-378"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(01)01186-X"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/38.11.1121"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.37.26272"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.282.1.62"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.11.5379-5387.1998"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2000.tb08942.x"},{"key":"e_1_3_2_9_2","first-page":"163","volume":"18","year":"1995","unstructured":"Fellinger, W., A. 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