{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,2]],"date-time":"2025-09-02T10:35:40Z","timestamp":1756809340277},"reference-count":21,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2002,2,1]],"date-time":"2002-02-01T00:00:00Z","timestamp":1012521600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Digene's Hybrid Capture 2 (HC2) CT\/GC, CT-ID, and GC-ID DNA tests were evaluated by comparison to traditional culture methods for detecting\n            <jats:italic>Chlamydia trachomatis<\/jats:italic>\n            and\n            <jats:italic>Neisseria gonorrhoeae<\/jats:italic>\n            infections in 669 cervical specimens from high-risk female populations attending two sexually transmitted disease clinics. For detection of either or both infections, the HC2 CT\/GC test algorithm had 93.8% sensitivity and 95.9% specificity compared to those of culture. After resolution of discrepant results by direct fluorescent-antibody (DFA) staining or PCR assay, the relative sensitivity and specificity of the HC2 CT\/GC test algorithm increased to 94.8 and 99.8%, while the values for culture were 83.6% (McNemar's\n            <jats:italic>P<\/jats:italic>\n            value, 0.0062) and 100%, respectively. For detection of the individual pathogens, the relative sensitivities for the HC2 CT-ID and GC-ID tests were 97.2 and 92.2% and the specificities were greater than 99% compared to culture adjucated by DFA staining and PCR. Test performance varied at the two clinics: the HC2 CT\/GC algorithm, CT-ID, and GC-ID tests had significantly higher sensitivities (McNemar's\n            <jats:italic>P<\/jats:italic>\n            value, &lt;0.05) than that of culture for the population at one clinic as well as for the combined populations. At the other clinic, the HC2 tests performed as well as culture.\n          <\/jats:p>","DOI":"10.1128\/jcm.40.02.641-644.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:53Z","timestamp":1027763753000},"page":"641-644","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["Comparison of Digene Hybrid Capture 2 and Conventional Culture for Detection of\n            <i>Chlamydia trachomatis<\/i>\n            and\n            <i>Neisseria gonorrhoeae<\/i>\n            in Cervical Specimens"],"prefix":"10.1128","volume":"40","author":[{"given":"Ling H.","family":"Darwin","sequence":"first","affiliation":[{"name":"Digene Corporation, Gaithersburg, Maryland 20878"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allison P.","family":"Cullen","sequence":"additional","affiliation":[{"name":"Digene Corporation, Gaithersburg, Maryland 20878"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick M.","family":"Arthur","sequence":"additional","affiliation":[{"name":"Digene Corporation, Gaithersburg, Maryland 20878"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carole D.","family":"Long","sequence":"additional","affiliation":[{"name":"Digene Corporation, Gaithersburg, Maryland 20878"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kim R.","family":"Smith","sequence":"additional","affiliation":[{"name":"University of Alabama at Birmingham, Birmingham, Alabama 35294"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jennifer L.","family":"Girdner","sequence":"additional","affiliation":[{"name":"Johns Hopkins University, Baltimore, Maryland 21205"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"III","given":"Edward W.","family":"Hook","sequence":"additional","affiliation":[{"name":"University of Alabama at Birmingham, Birmingham, Alabama 35294"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas C.","family":"Quinn","sequence":"additional","affiliation":[{"name":"Johns Hopkins University, Baltimore, Maryland 21205"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Attila T.","family":"Lorincz","sequence":"additional","affiliation":[{"name":"Digene Corporation, Gaithersburg, Maryland 20878"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.11.3023-3027.1993"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.10.1.160"},{"key":"e_1_3_2_4_2","first-page":"1","volume":"42","year":"1993","unstructured":"Centers for Disease Control. 1993. Sexually transmitted diseases treatment guidelines. Morb. Mortal. Wkly. Rep.42(RR-14):1-102.","journal-title":"Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.12.3111-3114.1995"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.6.1536-1540.1997"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.5.1579-1581.1999"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.10.2628-2633.1997"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.8.2129-2132.1997"},{"key":"e_1_3_2_10_2","first-page":"149","year":"1990","unstructured":"Hook, E. W., III, and H. H. Handsfield. 1990. Gonococcal infections in the adult, p. 149-165. In K. K. Holmes, P.-A. Mardh, P. F. Sparling, and P. J. Wiesner (ed.), Sexually transmitted diseases. 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