{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T08:29:46Z","timestamp":1792484986101,"version":"4.5.0"},"reference-count":16,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2002,1,1]],"date-time":"2002-01-01T00:00:00Z","timestamp":1009843200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Two outbreaks of hand-foot-and-mouth disease (HFMD) occurred in Taiwan between 1998 and 2000. Enteroviruses were isolated from a total of 1,892 patients in this laboratory during this period. Of the virus isolates, enterovirus 71 (EV71) was diagnosed in 44.4% of the patients (132 of 297) in 1998, 2% (13 of 646) in 1999, and 20.5% (195 of 949) in 2000. Genetic analyses of the 5\u2032-untranslated and VP1 regions of EV71 isolates by reverse transcription-PCR and sequencing were performed to understand the diversity of EV71 in these outbreaks of HFMD. Most EV71 isolates from the 1998 epidemic belonged to genotype C, while only one-tenth of the isolates were genotype B. Interestingly, all EV71 isolates tested from 1999 to 2000 belonged to genotype B. This study indicated that two genogroups of EV71 capable of inducing severe clinical illness have been circulating in Taiwan. Furthermore, the predominant EV71 genotypes responsible for each of the two major HFMD outbreaks within the 3-year period in Taiwan were different.<\/jats:p>","DOI":"10.1128\/jcm.40.1.10-15.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:03:40Z","timestamp":1027764220000},"page":"10-15","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":149,"title":["Change of Major Genotype of Enterovirus 71 in Outbreaks of Hand-Foot-and-Mouth Disease in Taiwan between 1998 and 2000"],"prefix":"10.1128","volume":"40","author":[{"given":"Jen-Ren","family":"Wang","sequence":"first","affiliation":[{"name":"Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan"},{"name":"Departments of Medical Technology, College of Medicine, National Cheng Kung University, Tainan, Taiwan"},{"name":"National Health Research Institutes Tainan Virology Laboratory for Diagnosis and Research, Department of Pathology, National Cheng Kung University Hospital, Tainan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yen-Chang","family":"Tuan","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huey-Pin","family":"Tsai","sequence":"additional","affiliation":[{"name":"National Health Research Institutes Tainan Virology Laboratory for Diagnosis and Research, Department of Pathology, National Cheng Kung University Hospital, Tainan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing-Jou","family":"Yan","sequence":"additional","affiliation":[{"name":"Pathology, College of Medicine, National Cheng Kung University, Tainan, Taiwan"},{"name":"National Health Research Institutes Tainan Virology Laboratory for Diagnosis and Research, Department of Pathology, National Cheng Kung University Hospital, Tainan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching-Chuan","family":"Liu","sequence":"additional","affiliation":[{"name":"Pediatrics, College of Medicine, National Cheng Kung University, Tainan, Taiwan"},{"name":"National Health Research Institutes Tainan Virology Laboratory for Diagnosis and Research, Department of Pathology, National Cheng Kung University Hospital, Tainan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ih-Jen","family":"Su","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine, College of Medicine, National Cheng Kung University, Tainan, Taiwan"},{"name":"Pathology, College of Medicine, National Cheng Kung University, Tainan, Taiwan"},{"name":"National Health Research Institutes Tainan Virology Laboratory for Diagnosis and Research, Department of Pathology, National Cheng Kung University Hospital, Tainan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"913","volume":"49","year":"2000","unstructured":"Anonymous. 2000. Enterovirus surveillance\u2014United States, 1997\u20131999. Morb. Mortal. Wkly. Rep.49:913\u2013916.","journal-title":"Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0168-1702(95)00087-9"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.73.12.9969-9975.1999"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199909233411301"},{"key":"e_1_3_2_6_2","first-page":"205","volume":"33","year":"2000","unstructured":"Ho, M. 2000. Enterovirus 71: the virus, its infections and outbreaks. J. Microbiol. Immunol. Infect.33:205\u2013216.","journal-title":"J. Microbiol. Immunol. Infect."},{"key":"e_1_3_2_7_2","first-page":"119","year":"1994","unstructured":"Hsiung, G. D. 1994. Picornaviridae, p.119\u2013140. In G. D. Hsiung, C. K. Y. Fong, and M. L. Landry (ed.), Hsiung\u2019s diagnostic virology, 4th ed. Yale University Press, New Haven, Conn.","journal-title":"Hsiung\u2019s diagnostic virology, 4th ed."},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1007\/s007050070102"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199512000-00013"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(00)00068-8"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.1.202"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/129.3.304"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-1702(00)00162-3"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.7883\/yoken.52.12"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(00)00185-3"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.1.111-118.2001"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(00)00079-2"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.40.1.10-15.2002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.40.1.10-15.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:00:17Z","timestamp":1646478017000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.40.1.10-15.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,1]]},"references-count":16,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2002,1]]}},"alternative-id":["10.1128\/JCM.40.1.10-15.2002"],"URL":"https:\/\/doi.org\/10.1128\/jcm.40.1.10-15.2002","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,1]]},"assertion":[{"value":"2001-04-30","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2001-10-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-01-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}