{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T04:19:06Z","timestamp":1787199546232,"version":"build-2736575974"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T00:00:00Z","timestamp":1033430400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            PCR-based approaches targeting kinetoplast DNA were evaluated for the diagnosis of American cutaneous leishmaniasis (ACL) in regions of endemicity in northeastern Brazil. A total of 119 cutaneous biopsy specimens from patients with ACL and nonleishmaniasis cutaneous lesions were studied. Two PCR-based systems were used; one was specific for the subgenus\n            <jats:italic>Viannia<\/jats:italic>\n            , and the other was specific for the genus\n            <jats:italic>Leishmania<\/jats:italic>\n            . The PCR specific for the subgenus\n            <jats:italic>Viannia<\/jats:italic>\n            had a sensitivity of 95.4%, whereas the genus-specific PCR detected the target DNA in 88.2% of the samples tested. The specificities of the assays, determined with samples from a group with nonleishmaniasis cutaneous lesions, was 100%. The results of the conventional tests indicate that the sensitivities of the PCR-based methods were significantly higher than those of smear examination, histological staining, and isolation by culture (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.05). Antibodies specific for\n            <jats:italic>Leishmania braziliensis<\/jats:italic>\n            were detected by indirect immunofluorescence in 82.9% of the patients tested. Parasites were isolated from 40 of 86 patients (46.5%). Sixty-seven percent of dermal scrapings and 66.2% of stained tissue sections were positive by microscopy. Amplified products from the subgenus-specific PCR hybridized with the\n            <jats:italic>Leishmania panamensis<\/jats:italic>\n            minicircle, confirming infection consistent with\n            <jats:italic>L. braziliensis<\/jats:italic>\n            . The evidence available at present incriminates\n            <jats:italic>L. braziliensis<\/jats:italic>\n            as the only causative agent of ACL in the state of Pernambuco in Brazil.\n          <\/jats:p>","DOI":"10.1128\/jcm.40.10.3572-3576.2002","type":"journal-article","created":{"date-parts":[[2002,9,28]],"date-time":"2002-09-28T00:04:10Z","timestamp":1033171450000},"page":"3572-3576","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":56,"title":["Evaluation of PCR for Diagnosis of American Cutaneous Leishmaniasis in an Area of Endemicity in Northeastern Brazil"],"prefix":"10.1128","volume":"40","author":[{"given":"Eduardo Henrique Gomes","family":"Rodrigues","sequence":"first","affiliation":[{"name":"Centro de Pesquisas Aggeu Magalha\u0303es, Fundac\u0327a\u0303o Oswaldo Cruz"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Edileuza","family":"Felinto de Brito","sequence":"additional","affiliation":[{"name":"Centro de Pesquisas Aggeu Magalha\u0303es, Fundac\u0327a\u0303o Oswaldo Cruz"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitzi Guedes","family":"Mendonc\u0327a","sequence":"additional","affiliation":[{"name":"Departmento de Dermatologia, Universidade Federal de Pernambuco, Recife, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto P.","family":"Werkha\u0308user","sequence":"additional","affiliation":[{"name":"Centro de Pesquisas Aggeu Magalha\u0303es, Fundac\u0327a\u0303o Oswaldo Cruz"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eridan M.","family":"Coutinho","sequence":"additional","affiliation":[{"name":"Centro de Pesquisas Aggeu Magalha\u0303es, Fundac\u0327a\u0303o Oswaldo Cruz"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wayner V.","family":"Souza","sequence":"additional","affiliation":[{"name":"Centro de Pesquisas Aggeu Magalha\u0303es, Fundac\u0327a\u0303o Oswaldo Cruz"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria de Fa\u0301tima P.","family":"Milita\u0303o de Albuquerque","sequence":"additional","affiliation":[{"name":"Centro de Pesquisas Aggeu Magalha\u0303es, Fundac\u0327a\u0303o Oswaldo Cruz"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u0301rcio L.","family":"Jardim","sequence":"additional","affiliation":[{"name":"Departmento de Dermatologia, Universidade Federal de Pernambuco, Recife, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederico G. 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