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Review of 21 retrospective and prospective hospital-acquired<jats:italic>E. coli<\/jats:italic>infections revealed that the isolates had similar antibiotic resistance profiles, characterized by resistance to most cephalosporins, \u03b2-lactams, and the \u03b2-lactamase inhibitor clavulanic acid as well as resistance to tetracycline, spectinomycin, sulfonamides, chloramphenicol, and gentamicin.<jats:italic>E. coli<\/jats:italic>isolates with similar resistance profiles were also isolated from the environment in the intensive care unit and surgery wards. Multiple<jats:italic>E. coli<\/jats:italic>genetic types were endemic to the hospital environment, with the pulsed-field gel electrophoresis fingerprint identified among<jats:italic>E. coli<\/jats:italic>isolates from diseased animals and the hospital environment matching. The extended-spectrum cephalosporin resistance in these nosocomial<jats:italic>E. coli<\/jats:italic>isolates was attributed to the cephamycinase-encoding gene,<jats:italic>bla<\/jats:italic><jats:sub>CMY2<\/jats:sub>. Chloramphenicol resistance was due in part to the dissemination of the florfenicol resistance gene,<jats:italic>flo<\/jats:italic>, among these isolates. Resistance encoded by both genes was self-transmissible. Although<jats:italic>bla<\/jats:italic><jats:sub>CMY2<\/jats:sub>and<jats:italic>flo<\/jats:italic>were common to the polyclonal, nosocomial<jats:italic>E. coli<\/jats:italic>isolates, there was considerable diversity in the genetic compositions of class 1 integrons, especially among isolates belonging to the same genetic type. Two or more integrons were generally present in these isolates. The gene cassettes present within each integron ranged in size from 0.6 to 2.4 kb, although a 1.7-kb gene cassette was the most prevalent. The 1.7-kb gene cassette contained spectinomycin resistance gene<jats:italic>aadA5<\/jats:italic>and trimethoprim resistance gene<jats:italic>dfrA17<\/jats:italic>.<\/jats:p>","DOI":"10.1128\/jcm.40.10.3586-3595.2002","type":"journal-article","created":{"date-parts":[[2002,9,28]],"date-time":"2002-09-28T00:04:10Z","timestamp":1033171450000},"page":"3586-3595","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":97,"title":["Characterization of Multidrug-Resistant<i>Escherichia coli<\/i>Isolates Associated with Nosocomial Infections in Dogs"],"prefix":"10.1128","volume":"40","author":[{"given":"Susan","family":"Sanchez","sequence":"first","affiliation":[{"name":"Athens Diagnostic Laboratory"},{"name":"the Departments of Medical Microbiology and Parasitology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. 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