{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T12:34:42Z","timestamp":1762864482430},"reference-count":15,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T00:00:00Z","timestamp":1033430400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We examined 73 recent invasive pneumococcal isolates within selected areas of Italy for genotypic variability. Thirty-three genomic macrorestriction types were found, three of which represented multiple serotypes. Restriction fragment patterns of\n            <jats:italic>pbp2b<\/jats:italic>\n            ,\n            <jats:italic>pbp2x<\/jats:italic>\n            , and\n            <jats:italic>pspA<\/jats:italic>\n            were conserved within the majority of isolates that shared macrorestriction types. Of the nine macrorestriction types found among the 22 penicillin-nonsusceptible\n            <jats:italic>Streptococus pneumoniae<\/jats:italic>\n            (PNSP) isolates, seven comprised isolates with allelic profiles showing five to seven allelic matches to profiles in the multilocus sequence typing database (\n            <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"url\" xlink:href=\"www.mlst.net\">www.mlst.net<\/jats:ext-link>\n            ); however, three of the seven profiles represented serotypes not previously associated with these clonal clusters. Two PNSP macrorestriction types represented new clones with unique allelic profiles. Allelic profiles obtained from isolates of 3 of the 25 macrorestriction types found among the 51 penicillin-susceptible\n            <jats:italic>S. pneumoniae<\/jats:italic>\n            (PSSP) isolates were closely related to previously described profiles. One PSSP isolate was a novel type 24F isolate related to the multiresistant clone France\n            <jats:sup>9V<\/jats:sup>\n            -3. This work reports new PNSP strains and new serotype-clone associations.\n          <\/jats:p>","DOI":"10.1128\/jcm.40.10.3660-3665.2002","type":"journal-article","created":{"date-parts":[[2002,9,28]],"date-time":"2002-09-28T00:04:10Z","timestamp":1033171450000},"page":"3660-3665","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":18,"title":["Genotypes of Invasive Pneumococcal Isolates Recently Recovered from Italian Patients"],"prefix":"10.1128","volume":"40","author":[{"given":"Giordano","family":"Dicuonzo","sequence":"first","affiliation":[{"name":"Dipartimento di Medicina di Laboratorio e Microbiologia, Universita\u0300 Campus Bio-Medico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giovanni","family":"Gherardi","sequence":"additional","affiliation":[{"name":"Dipartimento di Medicina di Laboratorio e Microbiologia, Universita\u0300 Campus Bio-Medico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert E.","family":"Gertz","sequence":"additional","affiliation":[{"name":"National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fabio","family":"D'Ambrosio","sequence":"additional","affiliation":[{"name":"Laboratorio di Batteriologia e Micologia Medica, Istituto Superiore di Sanita\u0300"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Antonio","family":"Goglio","sequence":"additional","affiliation":[{"name":"Laboratorio di Microbiologia, Ospedali Riuniti, Bergamo, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia","family":"Lorino","sequence":"additional","affiliation":[{"name":"Laboratorio di Microbiologia, Universita\u0300 La Sapienza, Rome"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simona","family":"Recchia","sequence":"additional","affiliation":[{"name":"Laboratorio di Batteriologia e Micologia Medica, Istituto Superiore di Sanita\u0300"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annalisa","family":"Pantosti","sequence":"additional","affiliation":[{"name":"Laboratorio di Batteriologia e Micologia Medica, Istituto Superiore di Sanita\u0300"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernard","family":"Beall","sequence":"additional","affiliation":[{"name":"National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.10.3663-3669.2000"},{"key":"e_1_3_2_3_2","first-page":"1","volume":"46","year":"1997","unstructured":"Centers for Disease Control and Prevention. 1997. 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Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard (M7-A5) 5th ed. National Committee for Clinical Laboratory Standards Wayne Pa."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1086\/317502"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1086\/341250"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.45.9.2648-2650.2001"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.40.10.3660-3665.2002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.40.10.3660-3665.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T01:22:58Z","timestamp":1646443378000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.40.10.3660-3665.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,10]]},"references-count":15,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2002,10]]}},"alternative-id":["10.1128\/JCM.40.10.3660-3665.2002"],"URL":"https:\/\/doi.org\/10.1128\/jcm.40.10.3660-3665.2002","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,10]]},"assertion":[{"value":"2002-05-28","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-07-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-10-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}