{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T09:16:26Z","timestamp":1767863786940,"version":"3.49.0"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T00:00:00Z","timestamp":1033430400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The gene encoding\n            <jats:italic>Babesia bovis<\/jats:italic>\n            rhoptry-associated protein 1 (RAP-1) was used to develop an enzyme-linked immunosorbent assay (ELISA) to measure specific antibodies against\n            <jats:italic>B. bovis.<\/jats:italic>\n            The\n            <jats:italic>B. bovis<\/jats:italic>\n            RAP-1 gene was subcloned into a baculovirus transfer vector, and the RAP-1 protein was expressed in insect cells infected with a recombinant baculovirus. The recombinant\n            <jats:italic>B. bovis<\/jats:italic>\n            RAP-1 of 65 kDa was detected with anti-RAP-1 mouse serum by Western blotting, and this recombinant RAP-1 was used as an antigen in the ELISA. The ELISA was able to differentiate between\n            <jats:italic>B. bovis<\/jats:italic>\n            -infected sera and\n            <jats:italic>B. bigemina<\/jats:italic>\n            -infected sera or noninfected normal bovine sera. The results demonstrate that the recombinant RAP-1 expressed in insect cells might be a useful antigen for the detection of antibodies to\n            <jats:italic>B. bovis.<\/jats:italic>\n          <\/jats:p>","DOI":"10.1128\/jcm.40.10.3771-3775.2002","type":"journal-article","created":{"date-parts":[[2002,9,28]],"date-time":"2002-09-28T00:04:10Z","timestamp":1033171450000},"page":"3771-3775","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Evaluation of an Enzyme-Linked Immunosorbent Assay with Recombinant Rhoptry-Associated Protein 1 Antigen against\n            <i>Babesia bovis<\/i>\n            for the Detection of Specific Antibodies in Cattle"],"prefix":"10.1128","volume":"40","author":[{"given":"Suthisak","family":"Boonchit","sequence":"first","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuenan","family":"Xuan","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naoaki","family":"Yokoyama","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Will L.","family":"Goff","sequence":"additional","affiliation":[{"name":"Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, Washington 99164-6630"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gale","family":"Wagner","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&amp;M University, College Station, Texas 77843"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ikuo","family":"Igarashi","sequence":"additional","affiliation":[{"name":"National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"141","volume":"64","year":"1997","unstructured":"Avarazed, A., D. 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