{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:49:43Z","timestamp":1790729383946,"version":"4.1.0"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2002,11,1]],"date-time":"2002-11-01T00:00:00Z","timestamp":1036108800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Methicillin-resistant\n                    <jats:italic>Staphylococcus aureus<\/jats:italic>\n                    (MRSA) is one of the most important causes of hospital infections worldwide. High-level resistance to methicillin is caused by the\n                    <jats:italic>mecA<\/jats:italic>\n                    gene, which encodes an alternative penicillin-binding protein, PBP 2a. To determine the clonal relationships between methicillin-susceptible\n                    <jats:italic>S. aureus<\/jats:italic>\n                    (MSSA) and MRSA, we typed 1,069\n                    <jats:italic>S. aureus<\/jats:italic>\n                    isolates (493 MSSA isolates and 576 MRSA isolates), collected mainly in North American and European hospitals between the 1960s and the year 2000, using pulsed-field gel electrophoresis and ribotyping. Of 10 widespread\n                    <jats:italic>S. aureus<\/jats:italic>\n                    lineages recognized, 8 had corresponding\n                    <jats:italic>mecA<\/jats:italic>\n                    -positive strains. Multiresistant MRSA strains are found in hospitals worldwide, while unrelated and more susceptible strains represent less than 1% of the MRSA population. This supports the hypothesis that horizontal transfer plays an important role in the dissemination of the\n                    <jats:italic>mecA<\/jats:italic>\n                    gene in the\n                    <jats:italic>S. aureus<\/jats:italic>\n                    population.\n                  <\/jats:p>","DOI":"10.1128\/jcm.40.11.3970-3975.2002","type":"journal-article","created":{"date-parts":[[2002,10,30]],"date-time":"2002-10-30T15:42:12Z","timestamp":1035992532000},"page":"3970-3975","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":191,"title":["<i>mecA<\/i>\n                    Gene Is Widely Disseminated in\n                    <i>Staphylococcus aureus<\/i>\n                    Population"],"prefix":"10.1128","volume":"40","author":[{"given":"C. L. C.","family":"Wielders","sequence":"first","affiliation":[{"name":"Eijkman-Winkler Institute for Microbiology, Infectious Diseases and Inflammation, University Medical Centre, 3584 CX Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A. C.","family":"Fluit","sequence":"additional","affiliation":[{"name":"Eijkman-Winkler Institute for Microbiology, Infectious Diseases and Inflammation, University Medical Centre, 3584 CX Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Brisse","sequence":"additional","affiliation":[{"name":"Eijkman-Winkler Institute for Microbiology, Infectious Diseases and Inflammation, University Medical Centre, 3584 CX Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Verhoef","sequence":"additional","affiliation":[{"name":"Eijkman-Winkler Institute for Microbiology, Infectious Diseases and Inflammation, University Medical Centre, 3584 CX Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. J.","family":"Schmitz","sequence":"additional","affiliation":[{"name":"Eijkman-Winkler Institute for Microbiology, Infectious Diseases and Inflammation, University Medical Centre, 3584 CX Utrecht, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0966-842X(94)90608-4"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.3.447"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.165.2.373-378.1986"},{"key":"e_1_3_2_5_2","first-page":"707","volume":"48","year":"1999","unstructured":"Centers for Disease Control and Prevention. 1999. Four pediatric deaths from community acquired methicillin-resistant S. aureus\u2014Minnesota and North Dakota, 1997-1999. Morb. Mortal. Wkly. Rep.48:707-710.","journal-title":"Morb. Mortal. Wkly. 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