{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T16:16:10Z","timestamp":1789834570295,"version":"4.0.1"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2002,11,1]],"date-time":"2002-11-01T00:00:00Z","timestamp":1036108800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Alveolar echinococcosis (AE) is the most potentially lethal parasitic zoonosis of the nontropical areas in the northern hemisphere, where cystic echinococcosis (CE) is also endemic. Both AE and CE are highly endemic in China, and both serologic detection of echinococcosis, either AE or CE, and differentiation of AE from CE are crucial problems. Evaluation of Western blot analysis (WB) and enzyme-linked immunosorbent assay (ELISA) for the Em18 antigen, using affinity-purified and recombinant Em18, was carried out \u201cblindly\u201d using 60 human sera from patients diagnosed in France. The results were compared with those obtained using a commercially available\n            <jats:italic>Echinococcus<\/jats:italic>\n            WB immunoglobulin G (IgG) kit developed in France. The Em18 WB and\n            <jats:italic>Echinococcus<\/jats:italic>\n            WB IgG showed very similar results for detection of AE. Both affinity-purified Em18 or a recombinant Em18 WB and\n            <jats:italic>Echinococcus<\/jats:italic>\n            WB IgG seem useful for identification of AE, and the latter seems appropriate for both AE and CE, whereas affinity-purified Em18 ELISA and the newly developed recombinant Em18 ELISA appear to be suitable for detection of AE, especially for epidemiological surveys.\n          <\/jats:p>","DOI":"10.1128\/jcm.40.11.4161-4165.2002","type":"journal-article","created":{"date-parts":[[2002,10,30]],"date-time":"2002-10-30T20:42:12Z","timestamp":1036010532000},"page":"4161-4165","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["Evaluation of an Enzyme-Linked Immunosorbent Assay (ELISA) with Affinity-Purified Em18 and an ELISA with Recombinant Em18 for Differential Diagnosis of Alveolar Echinococcosis: Results of a Blind Test"],"prefix":"10.1128","volume":"40","author":[{"given":"Akira","family":"Ito","sequence":"first","affiliation":[{"name":"Department of Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ning","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Parasitology"},{"name":"Sichuan Institute of Parasitic Diseases, Chengdu"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martine","family":"Liance","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Ho\u0302pital Henri Mondor AP-HP, et Universite\u0301 Paris 12, Paris"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marcello O.","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuhito","family":"Sako","sequence":"additional","affiliation":[{"name":"Department of Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wulamu","family":"Mamuti","sequence":"additional","affiliation":[{"name":"Department of Parasitology"},{"name":"Department of Parasitology, Xinjiang Medical University, Urumqi, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuji","family":"Ishikawa","sequence":"additional","affiliation":[{"name":"Department of Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minoru","family":"Nakao","sequence":"additional","affiliation":[{"name":"Department of Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Yamasaki","sequence":"additional","affiliation":[{"name":"Department of Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Nakaya","sequence":"additional","affiliation":[{"name":"Animal Laboratory for Medical Research, Asahikawa Medical College, Asahikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karine","family":"Bardonnet","sequence":"additional","affiliation":[{"name":"Universite\u0301 de Franche-Comte\u0301, WHO Collaborating Center on Prevention and Treatment of Human Echinococcosis, Besanc\u0327on, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Solange","family":"Bresson-Hadni","sequence":"additional","affiliation":[{"name":"Universite\u0301 de Franche-Comte\u0301, WHO Collaborating Center on Prevention and Treatment of Human Echinococcosis, Besanc\u0327on, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominique A.","family":"Vuitton","sequence":"additional","affiliation":[{"name":"Universite\u0301 de Franche-Comte\u0301, WHO Collaborating Center on Prevention and Treatment of Human Echinococcosis, Besanc\u0327on, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00042737-200012030-00011"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0001-706X(00)00134-0"},{"key":"e_1_3_2_4_2","first-page":"14","volume":"55","year":"1996","unstructured":"Gottstein, B., F. Rettens, A. J. Parkinson, and F. Wilson. 1996. Immunological parameters associated with susceptibility or resistance to alveolar hydatid disease in Yupiks\/Inupiats. Arctic Med. Res.55:14-19.","journal-title":"Arctic Med. Res."},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(93)90476-7"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1999.60.188"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1998.58.790"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.4.1.57-59.1997"},{"key":"e_1_3_2_9_2","unstructured":"Ito A. C. Urbani J. M. Qiu D. A. Vuitton D. C. Qiu D. D. Heath P. S. Craig Z. Feng and P. M. Schantz. Control of echinococcosis and cysticercosis: a public health challenge to international cooperation in China. Acta Trop. in press."},{"key":"e_1_3_2_10_2","unstructured":"Ito A. Y. Sako Y. Ishikawa M. Nakao K. Nakaya and H. Yamasaki. 2002. Differential serodiagnosis for alveolar echinococcosis by Em18-immunoblot and Em18-ELISA in Japan and China p. 147-155. In P. S. Craig and Z. S. Pawlowski (ed.) Cestode zoonoses: echinococcosis and cysticercosis. An emergent and global problem. IOS Press Amsterdam The Netherlands."},{"key":"e_1_3_2_11_2","first-page":"111","volume":"32","year":"2001","unstructured":"Ito, A., Y. Sako, Y. Ishikawa, M. Nakao, and K. Nakaya. 2001. Differential serodiagnosis of cystic and alveolar echinococcosis using native recombinant antigens in Japan. Southeast Asian J. Trop. Med. Public Health32(Suppl. 2):111-115.","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"e_1_3_2_12_2","unstructured":"Ito A. Y. Sako H. Yamasaki W. Mamuti K. Nakaya M. Nakao and Y. Ishikawa. Development of Em18-immunoblot and Em18-ELISA for specific diagnosis of alveolar echinococcosis. Acta Trop. in press."},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1995.52.41"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(01)90235-4"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0016-5085(95)90350-X"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.10.3718-3721.2000"},{"key":"e_1_3_2_17_2","unstructured":"Pawlowski Z. S. J. Eckert D. A. Vuitton R. W. Ammann P. Kern P. S. Craig K. F. Far F. De Rosa C. Filice B. Gottstein F. Grimm C. N. L. Macpherson N. Sato T. Todorov J. Uchino W. von Sinner and H. Wen. 2001. Echinococcosis in humans: clinical aspects diagnosis and treatment p. 20-71. In J. Eckert M. A. Gemmell F.-X. Meslin and Z. S. Pawlowski (ed.) WHO\/OIE manual on echinococcosis in humans and animals: a public health problem of global concern. Organisation Internationale des Epizooties Paris France."},{"key":"e_1_3_2_18_2","first-page":"211","volume":"33","year":"1999","unstructured":"Qiu, J., F. J. Liu, H. Wang, A. Ito, and P. M. Schantz. 1999. A survey of hydatid disease (echinococcosis) in Tibetan populations in China: correlation of ultrasound and radiologic imaging and serologic results. Int. Arch. Hydatidosis33:211-213.","journal-title":"Int. Arch. Hydatidosis"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.8.2760-2765.2002"},{"key":"e_1_3_2_20_2","unstructured":"Wen H. J. J. Chai J. C. Wang S. H. Wang X. Y. Wang X. H. Feng and H. X. Zhou. 2002. Hydatid control within a continental system in P.R. China p. 355-365. In P. S. Craig and Z. S. Pawlowski (ed.) Cestode zoonoses: echinococcosis and cysticercosis. An emergent and global problem. IOS Press Amsterdam The Netherlands."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.40.11.4161-4165.2002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.40.11.4161-4165.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T01:37:34Z","timestamp":1646444254000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.40.11.4161-4165.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,11]]},"references-count":19,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2002,11]]}},"alternative-id":["10.1128\/JCM.40.11.4161-4165.2002"],"URL":"https:\/\/doi.org\/10.1128\/jcm.40.11.4161-4165.2002","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,11]]},"assertion":[{"value":"2002-05-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-07-30","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}