{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,14]],"date-time":"2025-12-14T16:04:00Z","timestamp":1765728240140},"reference-count":19,"publisher":"American Society for Microbiology","issue":"11","license":[{"start":{"date-parts":[[2002,11,1]],"date-time":"2002-11-01T00:00:00Z","timestamp":1036108800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Mannoproteins are important and abundant structural components of fungal cell walls. The\n            <jats:italic>AFMP1<\/jats:italic>\n            gene encodes a cell wall galactomannoprotein of\n            <jats:italic>Aspergillus fumigatus<\/jats:italic>\n            . In the present study, we show that Afmp1p is secreted into the cell culture supernatant at a level that can be detected by Western blotting. A sensitive enzyme-linked immunosorbent assay (ELISA) developed with antibodies against Afmp1p was capable of detecting this protein from the cell culture supernatant of\n            <jats:italic>A. fumigatus<\/jats:italic>\n            . The anti-Afmp1p antibody is specific since it fails to react with any protein from lysates of\n            <jats:italic>Aspergillus flavus<\/jats:italic>\n            ,\n            <jats:italic>Aspergillus niger<\/jats:italic>\n            ,\n            <jats:italic>Aspergillus terreus<\/jats:italic>\n            ,\n            <jats:italic>Penicillium marneffei<\/jats:italic>\n            ,\n            <jats:italic>Candida albicans<\/jats:italic>\n            ,\n            <jats:italic>Cryptococcus neoformans<\/jats:italic>\n            ,\n            <jats:italic>Blastomyces dermatitidis<\/jats:italic>\n            , and\n            <jats:italic>Histoplasma capsulatum<\/jats:italic>\n            by Western blotting. In addition, this Afmp1p antigen-based ELISA is also specific for\n            <jats:italic>A. fumigatus<\/jats:italic>\n            since the cell culture supernatants of the other eight fungi gave negative results. Finally, a clinical evaluation of sera from invasive aspergillosis patients indicates that 8 of 15 (53%) patients are Afmp1p antigen test positive. Furthermore, an Afmp1p antibody test was performed with these serum specimens. The combined antibody and antigen tests for invasive aspergillosis carry a sensitivity of 86.7% (13 of 15). The specificities of the tests are high since none of the 138 control sera, including 100 from normal blood donors, 20 from patients with penicilliosis marneffei, 6 from patients with candidemia, 8 from patients with typhoid fever, and 4 from patients with melioidosis, was positive by either test. In conclusion, the combined Afmp1p antibody and antigen tests are highly sensitive and specific for\n            <jats:italic>A. fumigatus<\/jats:italic>\n            invasive aspergillosis.\n          <\/jats:p>","DOI":"10.1128\/jcm.40.11.4382-4387.2002","type":"journal-article","created":{"date-parts":[[2002,10,30]],"date-time":"2002-10-30T20:42:12Z","timestamp":1036010532000},"page":"4382-4387","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["Detection of Cell Wall Galactomannoprotein Afmp1p in Culture Supernatants of\n            <i>Aspergillus fumigatus<\/i>\n            and in Sera of Aspergillosis Patients"],"prefix":"10.1128","volume":"40","author":[{"given":"Patrick C. Y.","family":"Woo","sequence":"first","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Che-Man","family":"Chan","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andy S. P.","family":"Leung","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanna K. P.","family":"Lau","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-Yan","family":"Che","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samson S. Y.","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwok-Yung","family":"Yuen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital"},{"name":"HKU-Pasteur Research Centre, Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.3.966-973.1998"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.4.981-986.1999"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.10.3028-3031.1998"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.11.4211-4218.1995"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.6.2041-2045.2002"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/12.6.1147"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.7.2714-2719.1995"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.2.310"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.10.3223-3228.1999"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.12.5353-5360.1994"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.2.573-579.1995"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/166.5.1103"},{"key":"e_1_3_2_14_2","first-page":"1265","volume":"134","year":"1988","unstructured":"Scaringi, L., P. 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