{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,29]],"date-time":"2025-09-29T11:50:30Z","timestamp":1759146630118},"reference-count":23,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2002,12,1]],"date-time":"2002-12-01T00:00:00Z","timestamp":1038700800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Twenty-one\n            <jats:italic>Escherichia coli<\/jats:italic>\n            O157:H7 strains isolated in northern Italy from sporadic cases of hemolytic-uremic syndrome and from cattle and food were characterized by virulence gene analysis, pulsed-field gel electrophoresis (PFGE) of\n            <jats:italic>Xba<\/jats:italic>\n            I-digested DNA, enterobacterial repetitive intergenic consensus (ERIC) sequence-based PCR (ERIC-PCR), and antibiotic resistance patterns and compared to 18 strains isolated in France from human cases of diarrhea, cattle, and the environment. Strains isolated in Sicily (southern Italy) from a local farm (one strain) and from calves just imported from France (11 strains) and Spain (six strains) were also typed. Whereas the\n            <jats:italic>eae<\/jats:italic>\n            and\n            <jats:italic>hlyA<\/jats:italic>\n            genes were always detected, Shiga toxin gene (\n            <jats:italic>stx<\/jats:italic>\n            ) analysis showed some differences related to geographic areas. Isolates from northern Italy showed a high frequency of\n            <jats:italic>stx<\/jats:italic>\n            <jats:sub>1<\/jats:sub>\n            and\n            <jats:italic>stx<\/jats:italic>\n            <jats:sub>2<\/jats:sub>\n            , while strains isolated in France and from French and Spanish calves imported to Sicily more frequently possessed the\n            <jats:italic>stx<\/jats:italic>\n            <jats:sub>2c<\/jats:sub>\n            gene. The majority of the strains isolated in northern Italy were also resistant to one or more antibiotics, while most of the strains isolated in France and Sicily were fully susceptible. ERIC-PCR analysis was not able to differentiate the strains. PFGE typing after\n            <jats:italic>Xba<\/jats:italic>\n            I DNA digestion produced a total of 54 distinct restriction endonuclease digestion profiles (REDPs) among the 57 strains. Phylogenetic analysis was unable to cluster REDPs according to geographic origin. All epidemiologically related isolates showed either identical or \u226591% similar REDPs. Our findings suggest a peculiar circulation of antibiotic-resistant, genetically unrelated strains in northern Italy.\n          <\/jats:p>","DOI":"10.1128\/jcm.40.12.4619-4624.2002","type":"journal-article","created":{"date-parts":[[2002,11,26]],"date-time":"2002-11-26T22:43:39Z","timestamp":1038350619000},"page":"4619-4624","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Characterization of Shiga Toxin-Producing\n            <i>Escherichia coli<\/i>\n            O157:H7 Isolated in Italy and in France"],"prefix":"10.1128","volume":"40","author":[{"given":"Giovanni M.","family":"Giammanco","sequence":"first","affiliation":[{"name":"Dipartimento di Igiene e Microbiologia, Universita\u0300 di Palermo, I-90127 Palermo"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sarina","family":"Pignato","sequence":"additional","affiliation":[{"name":"Dipartimento G. F. Ingrassia, Igiene e Sanita\u0300 Pubblica, Universita\u0300 di Catania, I-95124 Catania"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francine","family":"Grimont","sequence":"additional","affiliation":[{"name":"Unite\u0301 Biodiversite\u0301 des Bacte\u0301ries Pathoge\u0300nes Emergentes, Institut Pasteur, F-75724 Paris Cedex 15, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick A. D.","family":"Grimont","sequence":"additional","affiliation":[{"name":"Unite\u0301 Biodiversite\u0301 des Bacte\u0301ries Pathoge\u0300nes Emergentes, Institut Pasteur, F-75724 Paris Cedex 15, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alfredo","family":"Caprioli","sequence":"additional","affiliation":[{"name":"Laboratorio di Medicina Veterinaria, Istituto Superiore di Sanita\u0300, I-00161 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefano","family":"Morabito","sequence":"additional","affiliation":[{"name":"Laboratorio di Medicina Veterinaria, Istituto Superiore di Sanita\u0300, I-00161 Rome, Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giuseppe","family":"Giammanco","sequence":"additional","affiliation":[{"name":"Dipartimento G. F. Ingrassia, Igiene e Sanita\u0300 Pubblica, Universita\u0300 di Catania, I-95124 Catania"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.epirev.a017914"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(98)80001-6"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.1.144-148.1996"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1135(99)00039-5"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199508103330608"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.60.7.2584-2592.1994"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/166.1.154"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268800057162"},{"key":"e_1_3_2_10_2","first-page":"301","volume":"44","year":"1997","unstructured":"Conedera, G., P. A. Marangon, A. Chapman, A. Zuin, and A. Caprioli. 1997. Atypical strains of verocytotoxin producing Escherichia coli O157 in beef cattle at slaughter in Veneto region. J. Vet. Med. B44:301-307.","journal-title":"J. Vet. Med. B"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.2307\/1932409"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.62.5.1519-1525.1996"},{"key":"e_1_3_2_13_2","first-page":"87","volume":"13","year":"2001","unstructured":"Giammanco, G. M., S. Pignato, A. M. Marino, R. P. Giunta, G. Faro, S. Caracappa, and G. Giammanco. 2001. Rischio di introduzione in Sicilia di Escherichia coli O157:H7, mediante bovini importati per la macellazione. Ann. Ig.13:87-92.","journal-title":"Ann. Ig."},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.epirev.a036079"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.12.3480-3487.1998"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.7.1675-1680.1997"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.6.2366-2368.2000"},{"key":"e_1_3_2_18_2","first-page":"1055","volume":"63","year":"1997","unstructured":"Schmidt, H., L. Beutin, and H. Karsch. 1997. Molecular analysis of the plasmid-encoded hemolysin of Escherichia coli O157:H7 strain EDL 933. Infect. Immun.63:1055-1061.","journal-title":"Infect. Immun."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.33.9.2233-2239.1995"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1991.tb00805.x"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.5.1180-1184.1998"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268800051645"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/19.24.6823"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1992.tb01484.x"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.40.12.4619-4624.2002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.40.12.4619-4624.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T01:24:16Z","timestamp":1646443456000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.40.12.4619-4624.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,12]]},"references-count":23,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2002,12]]}},"alternative-id":["10.1128\/JCM.40.12.4619-4624.2002"],"URL":"https:\/\/doi.org\/10.1128\/jcm.40.12.4619-4624.2002","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,12]]},"assertion":[{"value":"2002-07-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-09-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-12-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}