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The approach used in this study involves one-tube multiplex PCR amplification of six target bacterial virulence factor genes (<jats:italic>iap<\/jats:italic>,<jats:italic>hly<\/jats:italic>,<jats:italic>inlB<\/jats:italic>,<jats:italic>plcA<\/jats:italic>,<jats:italic>plcB<\/jats:italic>, and<jats:italic>clpE<\/jats:italic>), synthesis of fluorescently labeled single-stranded DNA, and hybridization to the multiple individual oligonucleotide probes specific for each<jats:italic>Listeria<\/jats:italic>species and immobilized on a glass surface. Results of the microarray analysis of 53 reference and clinical isolates of<jats:italic>Listeria<\/jats:italic>spp. demonstrated that this method allowed unambiguous identification of all six<jats:italic>Listeria<\/jats:italic>species based on sequence differences in the<jats:italic>iap<\/jats:italic>gene. Another virulence factor gene,<jats:italic>hly<\/jats:italic>, was used for detection and genotyping all<jats:italic>L. monocytogenes<\/jats:italic>, all<jats:italic>L. ivanovii<\/jats:italic>, and 8 of 11<jats:italic>L. seeligeri<\/jats:italic>isolates. Other members of the genus<jats:italic>Listeria<\/jats:italic>and three<jats:italic>L. seeligeri<\/jats:italic>isolates did not contain the<jats:italic>hly<\/jats:italic>gene. There was complete agreement between the results of genotyping based on the<jats:italic>hly<\/jats:italic>and<jats:italic>iap<\/jats:italic>gene sequences. All<jats:italic>L. monocytogenes<\/jats:italic>isolates were found to be positive for the<jats:italic>inlB<\/jats:italic>,<jats:italic>plcA<\/jats:italic>,<jats:italic>plcB<\/jats:italic>, and<jats:italic>clpE<\/jats:italic>virulence genes specific only to this species. Our data on<jats:italic>Listeria<\/jats:italic>species analysis demonstrated that this microarray technique is a simple, rapid, and robust genotyping method that is also a potentially valuable tool for identification and characterization of bacterial pathogens in general.<\/jats:p>","DOI":"10.1128\/jcm.40.12.4720-4728.2002","type":"journal-article","created":{"date-parts":[[2002,11,26]],"date-time":"2002-11-26T22:43:39Z","timestamp":1038350619000},"page":"4720-4728","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":177,"title":["Identification of<i>Listeria<\/i>Species by Microarray-Based Assay"],"prefix":"10.1128","volume":"40","author":[{"given":"Dmitriy","family":"Volokhov","sequence":"first","affiliation":[{"name":"Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, Maryland 20740-3835"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avraham","family":"Rasooly","sequence":"additional","affiliation":[{"name":"Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, Maryland 20740-3835"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Konstantin","family":"Chumakov","sequence":"additional","affiliation":[{"name":"Center for Biologics Evaluation and Research, Food and Drug Administration, Rockville, Maryland 20895"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Chizhikov","sequence":"additional","affiliation":[{"name":"Center for Biologics Evaluation and Research, Food and Drug Administration, Rockville, Maryland 20895"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"189","volume":"200","year":"1997","unstructured":"Allerberger, F., M. 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