{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T01:58:08Z","timestamp":1785895088697,"version":"3.56.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2002,4,1]],"date-time":"2002-04-01T00:00:00Z","timestamp":1017619200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Adverse effects of strain persistence and secretion in milk have been encountered with the\n            <jats:italic>Brucella melitensis<\/jats:italic>\n            vaccine strain Rev.1. Field isolates obtained from vaccinated animals and from a human resembled the vaccine strain Rev.1 by conventional bacteriological tests. The lack of a specific molecular marker that could specifically characterize the commercial vaccine strain prevented confirmation of the homology of the Rev.1-like field isolates to the vaccine strain. The composition of the\n            <jats:italic>omp2<\/jats:italic>\n            locus from two gene copies with differences in their\n            <jats:italic>Pst<\/jats:italic>\n            I restriction endonuclease sites was used to establish an epidemiologic fingerprint for the\n            <jats:italic>omp2<\/jats:italic>\n            gene in the Rev.1 vaccine strain. Primers designed to amplify DNA sequences that overlap the\n            <jats:italic>Pst<\/jats:italic>\n            I site revealed a single 282-bp DNA band common to all\n            <jats:italic>Brucella<\/jats:italic>\n            spp. Agarose gel electrophoresis of the\n            <jats:italic>Pst<\/jats:italic>\n            I digests of the PCR products from strains 16M and the vaccine strain Rev.1 revealed a distinctive profile that included three bands: one band for the intact 282-bp fragment amplified from\n            <jats:italic>omp2a<\/jats:italic>\n            and two bands resulting from the digestion of the amplified\n            <jats:italic>omp2b<\/jats:italic>\n            gene fragment, 238- and 44-bp DNA fragments, respectively. Amplified fragments of 37 Rev.1-like isolates, including 2 human isolates, also exhibited this pattern. In contrast, DNA digests of all other Israeli field isolates, including atypical\n            <jats:italic>B. melitensis<\/jats:italic>\n            biotype 1 and representatives of the biotype 2 and 3 isolates, produced two bands of 238 and 44 bp, respectively, corresponding with the digestion of both\n            <jats:italic>omp2a<\/jats:italic>\n            and\n            <jats:italic>omp2b<\/jats:italic>\n            genes. This method facilitates identification of the Rev.1 vaccine strain in both animals and humans in Israel.\n          <\/jats:p>","DOI":"10.1128\/jcm.40.2.1475-1480.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:59:40Z","timestamp":1027763980000},"page":"1475-1480","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":52,"title":["Identification of the\n            <i>Brucella melitensis<\/i>\n            Vaccine Strain Rev.1 in Animals and Humans in Israel by PCR Analysis of the\n            <i>Pst<\/i>\n            I Site Polymorphism of Its\n            <i>omp2<\/i>\n            Gene"],"prefix":"10.1128","volume":"40","author":[{"given":"Svetlana","family":"Bardenstein","sequence":"first","affiliation":[{"name":"Department of Bacteriology, Kimron Veterinary Institute, Bet Dagan 50250, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal","family":"Mandelboim","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Kimron Veterinary Institute, Bet Dagan 50250, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas A.","family":"Ficht","sequence":"additional","affiliation":[{"name":"Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&amp;M University, College Station, Texas 77843-4467"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam","family":"Baum","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Kimron Veterinary Institute, Bet Dagan 50250, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Menachem","family":"Banai","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, Kimron Veterinary Institute, Bet Dagan 50250, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"24","year":"1988","unstructured":"Alton, G. G., L. M. Jones, R. D. Angus, and J.-M. Verger (ed.). 1988. Techniques for the brucellosis laboratory, p. 24-61. Institut National de la Recherche Agronomique, Paris, France.","journal-title":"Techniques for the brucellosis laboratory"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.5.1057-1059.1990"},{"key":"e_1_3_2_4_2","unstructured":"Banai M. G. Hoida E. Rapoport O. Zamir M. Haimovich E. Katz I. Mayer A. Cohen and M. Davidson. 1991. Is Brucella melitensis undergoing evolutionary development or a change in the vaccinal Rev.1 strain? p. 152. In A. Tumbay S. Hilmi and O. Ang (ed.) Brucella and brucellosis in man and animals. Turkish Microbiological Society publication no. 16. Ege University Press Izmir Turkey."},{"key":"e_1_3_2_5_2","first-page":"69","year":"1996","unstructured":"Banai, M., M. Abramson, I. Mayer, K. Chechik, G. Hoida, O. Zamir, S. Bardenstein, A. Cohen, and M. Davidson. 1996. Problems associated with the persistence and possible horizontal transfer of Brucella melitensis Rev.1 vaccine in connection with serological surveillance in Israel, p. 69-76. In B. Garin-Bastugi and A. Benkirane (ed.), FAO\/WHO\/OIE Round Table on the Use of Rev.1 Vaccine in Small Ruminants and Cattle. Centre National d\u2019Etudes V\u00e9t\u00e9rinaires et Alimentaires, Maison-Alfort, France.","journal-title":"FAO\/WHO\/OIE Round Table on the Use of Rev.1 Vaccine in Small Ruminants and Cattle"},{"key":"e_1_3_2_6_2","first-page":"229","year":"1985","unstructured":"Bosseray, N. 1985. Quality control of four Rev.1 anti-Brucella vaccines, p. 229-236. In J. M. Verger and M. Plommet (ed.), Brucella melitensis. Martinus Nijhoff Publishers, Dordrecht, The Netherlands.","journal-title":"Brucella melitensis. Martinus Nijhoff Publishers"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1045-1056(05)80025-9"},{"key":"e_1_3_2_8_2","first-page":"121","volume":"79","year":"1992","unstructured":"Bosseray, N. 1992. Control methods and thresholds of acceptability for anti-Brucella vaccines. Dev. Biol. Stand.79:121-128.","journal-title":"Dev. Biol. Stand."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.11.2660-2666.1994"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1099\/13500872-141-9-2111"},{"key":"e_1_3_2_11_2","first-page":"1193","volume":"66","year":"1996","unstructured":"Elberg, S. 1996. Rev.1 Brucella melitensis vaccine. Part III, 1981-1995. Vet. Bull.66:1193-1200.","journal-title":"Vet. Bull."},{"key":"e_1_3_2_12_2","unstructured":"A guide to the diagnosis treatment and prevention of human brucellosis. World Health Organization publication VPH\/81.31 1981"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.73.2.211-217.1957"},{"key":"e_1_3_2_14_2","first-page":"711","volume":"19","year":"1958","unstructured":"Elberg, S. S., and K. F. Meyer. 1958. Caprine immunization against brucellosis. A summary of experiments on the isolation, properties and behaviour of a vaccine strain. Bull. W. H. O.19:711-724.","journal-title":"Bull. W. H. O."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1177\/104063879200400118"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.174.23.7778-7783.1992"},{"key":"e_1_3_2_17_2","first-page":"185","volume":"13","year":"1982","unstructured":"Fensterbank, R., P. Pardon, and J. Marly. 1982. Comparison between subcutaneous and conjunctival route of vaccination with Rev.1 strain against B. melitensis infection in ewes. Ann. Rech. Vet.13:185-190.","journal-title":"Ann. Rech. Vet."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1990.tb00688.x"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.56.8.2036-2046.1988"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.57.11.3281-3291.1989"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-46-1-329"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1136\/vr.147.22.632"},{"key":"e_1_3_2_23_2","first-page":"92","volume":"60","year":"1989","unstructured":"Hunter, P., S. M. Pefanis, C. C. Williamson, W. J. S. Botha, and M. S. Schalkwyk. 1989. Horizontal transmission in sheep and delayed clearance in guinea pigs and mice of a Brucella melitensis Rev.1 mutant. J. S. Afr. Vet. Assoc.60:92-94.","journal-title":"1 mutant. J. S. Afr. Vet. Assoc."},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.12.3087-3090.1995"},{"key":"e_1_3_2_25_2","first-page":"73","volume":"56","year":"1984","unstructured":"Meyer, M. E. 1984. Inter- and intra-strain variants in the genus Brucella. Dev. Biol. Stand.56:73-83.","journal-title":"Dev. Biol. Stand."},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.12.4163-4166.1999"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(87)55023-6"},{"key":"e_1_3_2_28_2","first-page":"181","volume":"55","year":"1988","unstructured":"Pefanis, S. M., B. Gummow, P. M. Pieterson, C. Williamson, C. G. Venter, and S. Herr. 1988. The isolation and serology of the \u201cFSA\u201d Brucella melitensis Rev.1 mutant in a flock of sheep. Onderstepoort J. Vet. Res.55:181-183.","journal-title":"Onderstepoort J. Vet. Res."},{"key":"e_1_3_2_29_2","first-page":"15","volume":"55","year":"1988","unstructured":"Pieterson, P. M., B. Gummow, S. 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