{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T18:48:04Z","timestamp":1791053284264,"version":"4.1.0"},"reference-count":51,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2002,2,1]],"date-time":"2002-02-01T00:00:00Z","timestamp":1012521600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Bartonella koehlerae<\/jats:italic>\n                    , a recently described feline\n                    <jats:italic>Bartonella<\/jats:italic>\n                    species, was isolated from two naturally infected cats in northern California. We experimentally infected domestic cats with\n                    <jats:italic>B. koehlerae<\/jats:italic>\n                    to establish the microbiological and immunological characteristics of this infection in cats and to compare it to infections with those caused by\n                    <jats:italic>B. henselae<\/jats:italic>\n                    and\n                    <jats:italic>B. clarridgeiae<\/jats:italic>\n                    . Four cats were inoculated intradermally with\n                    <jats:italic>B. koehlerae<\/jats:italic>\n                    (8.6 \u00d7 10\n                    <jats:sup>7<\/jats:sup>\n                    to 3.84 \u00d7 10\n                    <jats:sup>8<\/jats:sup>\n                    CFU\/ml). None of the cats presented any obvious clinical signs, but all cats developed bacteremia, which peaked at 3.36 \u00d7 10\n                    <jats:sup>4<\/jats:sup>\n                    to 1.44 \u00d7 10\n                    <jats:sup>6<\/jats:sup>\n                    CFU\/ml of blood between day 14 and day 36 postinoculation.\n                    <jats:italic>B. koehlerae<\/jats:italic>\n                    -inoculated cats had a bacteremia duration (mean, 74 days) shorter than did cats inoculated with\n                    <jats:italic>B. clarridgeiae<\/jats:italic>\n                    (mean, 324 days) (\n                    <jats:italic>P<\/jats:italic>\n                    = 0.03). None of the four cats inoculated with\n                    <jats:italic>B. koehlerae<\/jats:italic>\n                    had bacteremia relapse. As shown by enzyme-linked immunosorbent assay (ELISA) using\n                    <jats:italic>B. koehlerae<\/jats:italic>\n                    outer membrane protein (OMP) antigens, the four cats developed a species-specific antibody response, and ELISA testing using other feline\n                    <jats:italic>Bartonella<\/jats:italic>\n                    OMP antigens showed statistically lower optical density values. All four cats developed similar antibody reactivity patterns to\n                    <jats:italic>B. koehlerae<\/jats:italic>\n                    OMP antigens as seen by Western blotting, each with at least 20 seroreactive protein bands. Using sodium dodecyl sulfate-polyacrylamide gel electrophoresis, protein profile differences were observed for both whole-cell lysate and OMPs from\n                    <jats:italic>B. koehlerae<\/jats:italic>\n                    , compared with\n                    <jats:italic>B. henselae<\/jats:italic>\n                    and\n                    <jats:italic>B. clarridgeiae. B. koehlerae<\/jats:italic>\n                    was more closely related to\n                    <jats:italic>B. henselae<\/jats:italic>\n                    than to\n                    <jats:italic>B. clarridgeiae<\/jats:italic>\n                    by protein profile, and this relatedness was also confirmed by analysis of the genomic DNA profiles by pulsed-field gel electrophoresis.\n                  <\/jats:p>","DOI":"10.1128\/jcm.40.2.466-474.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T05:55:53Z","timestamp":1027749353000},"page":"466-474","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":32,"title":["Experimental Infection of Domestic Cats with\n                    <i>Bartonella koehlerae<\/i>\n                    and Comparison of Protein and DNA Profiles with Those of Other\n                    <i>Bartonella<\/i>\n                    Species Infecting Felines"],"prefix":"10.1128","volume":"40","author":[{"given":"Kazuhiro","family":"Yamamoto","sequence":"first","affiliation":[{"name":"Department of Population Health and Reproduction"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno B.","family":"Chomel","sequence":"additional","affiliation":[{"name":"Department of Population Health and Reproduction"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rickie W.","family":"Kasten","sequence":"additional","affiliation":[{"name":"Department of Population Health and Reproduction"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carrie M.","family":"Hew","sequence":"additional","affiliation":[{"name":"Department of Population Health and Reproduction"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David K.","family":"Weber","sequence":"additional","affiliation":[{"name":"Center for Companion Animal Health, School of Veterinary Medicine, University of California, Davis, California 95616"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilson I.","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Population Health and Reproduction"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Droz","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology, University of Berne, CH-3010 Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jane E.","family":"Koehler","sequence":"additional","affiliation":[{"name":"and Division of Infectious Diseases, Department of Medicine, University of California, San Francisco, California 94143-0654"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0147-9571(96)00025-2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.2.743-746.2001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.9.2256-2261.1997"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.2.254-260.1996"},{"key":"e_1_3_2_6_2","unstructured":"Int. 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