{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T13:59:08Z","timestamp":1791295148270,"version":"4.1.0"},"reference-count":42,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2002,2,1]],"date-time":"2002-02-01T00:00:00Z","timestamp":1012521600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Periodontitis is a common chronic oral infection caused by gram-negative bacteria, including\n            <jats:italic>Actinobacillus actinomycetemcomitans<\/jats:italic>\n            and\n            <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n            . Periodontitis evokes inflammatory host response locally in the periodontium but also systemically. The systemic humoral antibody response against oral pathogens can conveniently be measured by an immunoassay. The aim of the study was to measure serum immunoglobulin G class antibodies against\n            <jats:italic>A. actinomycetemcomitans<\/jats:italic>\n            and\n            <jats:italic>P. gingivalis<\/jats:italic>\n            by an enzyme-linked immunosorbent assay (ELISA) in which mixtures of several serotypes of the pathogens were used as antigens to avoid biasing of the results in favor of a particular strain. For\n            <jats:italic>A. actinomycetemcomitans<\/jats:italic>\n            the antigen consisted of six strains representing serotypes a, b, c, d, and e and one nonserotypeable strain. In the\n            <jats:italic>P. gingivalis<\/jats:italic>\n            ELISA, antigens representing serotypes a, b, and c were used. Serum samples from 90 subjects, including 35 samples from patients with diagnosed periodontitis, 10 samples from periodontally healthy controls, and 45 samples from randomly selected apparently healthy volunteers (referred to as \u201chealthy subjects\u201d), were tested. For both pathogens the antibody levels (means \u00b1 standard deviations) of the patients\u2014expressed as area under the dilution curve\u2014were significantly higher than those for healthy controls or healthy subjects, with values for\n            <jats:italic>A. actinomycetemcomitans<\/jats:italic>\n            and\n            <jats:italic>P. gingivalis<\/jats:italic>\n            , respectively, as follows: patients, 22.60 \u00b1 9.94 mm\n            <jats:sup>2<\/jats:sup>\n            and 26.72 \u00b1 11.13 mm\n            <jats:sup>2<\/jats:sup>\n            ; healthy controls, 9.99 \u00b1 3.92 mm\n            <jats:sup>2<\/jats:sup>\n            and 6.90 \u00b1 3.38 mm\n            <jats:sup>2<\/jats:sup>\n            ; and healthy subjects, 16.85 \u00b1 6.67 mm\n            <jats:sup>2<\/jats:sup>\n            and 8.51 \u00b1 4.23 mm\n            <jats:sup>2<\/jats:sup>\n            . The serotype mixture ELISA is suitable for measuring antibodies against periodontal pathogens in large epidemiological studies in order to evaluate the role of periodontitis as a risk factor for other diseases.\n          <\/jats:p>","DOI":"10.1128\/jcm.40.2.512-518.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:58:35Z","timestamp":1027763915000},"page":"512-518","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":76,"title":["Multiserotype Enzyme-Linked Immunosorbent Assay as a Diagnostic Aid for Periodontitis in Large-Scale Studies"],"prefix":"10.1128","volume":"40","author":[{"given":"P. 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