{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,27]],"date-time":"2025-10-27T04:46:16Z","timestamp":1761540376236},"reference-count":29,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2002,2,1]],"date-time":"2002-02-01T00:00:00Z","timestamp":1012521600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Recently, we reported that the patterns of antibodies to\n            <jats:italic>Helicobacter pylori<\/jats:italic>\n            protein antigens in serum may be useful for screening patients at high risk for ulcers (P. Aucher et al., J. Clin. Microbiol. 36:931-936, 1998). Here we report the identification, by a combination of electrophoretic, immunochemical, and protein sequencing methods, of five antigens that correspond to this antibody pattern: groEL, catalase A, flagellin A, beta-ketoacyl-acyl carrier protein synthase I (\u03b2-ketoacyl-ACP S), and peptidyl prolyl\n            <jats:italic>cis-trans<\/jats:italic>\n            isomerase (PPiase). \u03b2-Ketoacyl-ACP S and PPiase are reported for the first time as antigens of diagnostic interest in infections by\n            <jats:italic>H. pylori<\/jats:italic>\n            . The antigenicity of the five antigens, together with those of CagA and VacA, was tested in an immunoblot assay with water-soluble protein extracts from two\n            <jats:italic>H. pylori<\/jats:italic>\n            pathogenic strains (HP 141 and ATCC 43579) and panels of sera from\n            <jats:italic>H. pylori<\/jats:italic>\n            -positive patients with gastroduodenal ulcers (GDU), nonulcer dyspepsia (NUD), as well as sera from\n            <jats:italic>H. pylori<\/jats:italic>\n            -negative healthy volunteers. For catalase A, groEL, and flagellin A antigens, no overall statistically important values were found making it possible to discriminate between patients with GDU and NUD. For both\n            <jats:italic>H. pylori<\/jats:italic>\n            strains, the mean performance indices (MPI) presenting percentages of correctly classified patients with GDU and NUD showed that the most significant antibody patterns were as follows: anti-VacA + anti-\u03b2-ketoacyl-ACP S (MPI = 76.1), anti-VacA + anti-PPiase (MPI = 71.8), and anti-CagA + anti-VacA + anti-\u03b2-ketoacyl-ACP S (MPI = 70.5). Antibody patterns detected with these antigen profiles may therefore be useful in developing a diagnostic test designed to predict the clinical severity of the\n            <jats:italic>H. pylori<\/jats:italic>\n            infection within the adult population of France.\n          <\/jats:p>","DOI":"10.1128\/jcm.40.2.547-552.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:53Z","timestamp":1027763753000},"page":"547-552","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Novel Antigens of\n            <i>Helicobacter pylori<\/i>\n            Correspond to Ulcer-Related Antibody Pattern of Sera from Infected Patients"],"prefix":"10.1128","volume":"40","author":[{"given":"Christo","family":"Atanassov","sequence":"first","affiliation":[{"name":"Department of Microbiology A, IFR 59, University Hospital Center, 86021 Poitiers"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leon","family":"Pezennec","sequence":"additional","affiliation":[{"name":"Department of Microbiology A, IFR 59, University Hospital Center, 86021 Poitiers"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacques","family":"d'Alayer","sequence":"additional","affiliation":[{"name":"Laboratory of Protein Microsequencing, Institut Pasteur, 75724 Paris"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ghislaine","family":"Grollier","sequence":"additional","affiliation":[{"name":"Department of Microbiology A, IFR 59, University Hospital Center, 86021 Poitiers"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bertrand","family":"Picard","sequence":"additional","affiliation":[{"name":"Laboratory of Microbiology, Faculty of Medicine, 29200 Brest, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jean-Louis","family":"Fauche\u0300re","sequence":"additional","affiliation":[{"name":"Department of Microbiology A, IFR 59, University Hospital Center, 86021 Poitiers"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/16495"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.4.931-936.1998"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M910233199"},{"key":"e_1_3_2_5_2","first-page":"170","volume":"105","year":"1997","unstructured":"Chatha, L., P. 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