{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,24]],"date-time":"2026-07-24T13:55:40Z","timestamp":1784901340851,"version":"3.55.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2002,2,1]],"date-time":"2002-02-01T00:00:00Z","timestamp":1012521600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Extended-spectrum \u03b2-lactamases (ESBLs) are widespread in hospital settings worldwide. The present investigation was undertaken to assess the distribution and prevalence of ESBLs belonging to the TEM and SHV families in 448 ESBL-producing clinical isolates of\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            collected from 10 different Italian hospitals. The natures of TEM and SHV determinants were identified by direct sequencing of PCR-amplified genes. TEM-52 and SHV-12 were the most common variants, and they were found in most hospitals and in several different species. Other less frequent variants included TEM-5, TEM-12, TEM-15, TEM-19, TEM-20, TEM-24, TEM-26, TEM-43, TEM-60, TEM-72, TEM-87, SHV-2a, SHV-5, and SHV-11.\n            <jats:italic>Proteus mirabilis<\/jats:italic>\n            was the most common producer of TEM-type ESBLs, while\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            was the most common producer of SHV-type ESBLs. The distribution of TEM- and SHV-type ESBL variants in\n            <jats:italic>Enterobacteriaceae<\/jats:italic>\n            from Italian hospitals exhibited notable differences from those from other geographical settings.\n          <\/jats:p>","DOI":"10.1128\/jcm.40.2.611-614.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:53Z","timestamp":1027763753000},"page":"611-614","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":96,"title":["Molecular Characterization of Extended-Spectrum \u03b2-Lactamases Produced by Nosocomial Isolates of\n            <i>Enterobacteriaceae<\/i>\n            from an Italian Nationwide Survey"],"prefix":"10.1128","volume":"40","author":[{"given":"Mariagrazia","family":"Perilli","sequence":"first","affiliation":[{"name":"Dipartimento di Scienze e Tecnologie Biomediche"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emanuela","family":"Dell'Amico","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Universita\u0300 di Siena, Siena"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernardetta","family":"Segatore","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze e Tecnologie Biomediche"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Rosaria","family":"de Massis","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze e Tecnologie Biomediche"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ciro","family":"Bianchi","sequence":"additional","affiliation":[{"name":"Dipartimento MedicoWyeth-Lederle Italia S.p.A., Aprilia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesco","family":"Luzzaro","sequence":"additional","affiliation":[{"name":"Ospedale di Circolo di Varese, Universita\u0300 dell' Insubria, Varese"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gian Maria","family":"Rossolini","sequence":"additional","affiliation":[{"name":"Dipartimento di Biologia Molecolare, Sezione di Microbiologia, Universita\u0300 di Siena, Siena"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Toniolo","sequence":"additional","affiliation":[{"name":"Ospedale di Circolo di Varese, Universita\u0300 dell' Insubria, Varese"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppe","family":"Nicoletti","sequence":"additional","affiliation":[{"name":"Universita\u0300 di Catania, Catania, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianfranco","family":"Amicosante","sequence":"additional","affiliation":[{"name":"Dipartimento di Scienze e Tecnologie Biomediche"},{"name":"Cattedra di Biochimica Clinica, Universita\u0300 di L'Aquila, L'Aquila"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.4.761"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/43.4.447"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/38.1.127"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/27.4.441"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.11.3177-3179.2000"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.42.6.1459"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0891-5520(05)70395-0"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/10.4.867"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.5.1446-1449.1998"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.39.12.2593"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/45.3.349"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.8.4.557"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/0147-619X(90)90041-A"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01591363"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.5.943"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s100960000363"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.6.1758-1763.1999"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/44.4.489"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.41.11.2374"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.9.2537-2539.2000"},{"key":"e_1_3_2_22_2","unstructured":"Perilli M. B. Segatore M. R. De Massis N. Franceschini C. Bianchi G. M. Rossolini and G. Amicosante. Characterization of a new extended-spectrum \u03b2-lactamase (TEM-87) isolated in Proteus mirabilis during an Italian survey. Antimicrob. 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