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When applied to 24 pneumococcal DNA-positive cerebrospinal fluid extracts, penicillin-sensitive<jats:italic>S. pneumoniae<\/jats:italic>was detected in all instances. Real-time PCR proved more sensitive than culture, microscopy, or antigen detection and provided susceptibility data even in culture-negative cases.<\/jats:p>","DOI":"10.1128\/jcm.40.2.682-684.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:55:53Z","timestamp":1027763753000},"page":"682-684","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":26,"title":["Rapid Real-Time PCR for Determination of Penicillin Susceptibility in Pneumococcal Meningitis, Including Culture-Negative Cases"],"prefix":"10.1128","volume":"40","author":[{"given":"Angela M.","family":"Kearns","sequence":"first","affiliation":[{"name":"Public Health Laboratory, Newcastle General Hospital, Newcastle upon Tyne NE4 6BE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Clive","family":"Graham","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Royal Victoria Infirmary, Newcastle upon Tyne NE1 4LP, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Doreen","family":"Burdess","sequence":"additional","affiliation":[{"name":"Public Health Laboratory, Newcastle General Hospital, Newcastle upon Tyne NE4 6BE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane","family":"Heatherington","sequence":"additional","affiliation":[{"name":"Public Health Laboratory, Newcastle General Hospital, Newcastle upon Tyne NE4 6BE"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roger","family":"Freeman","sequence":"additional","affiliation":[{"name":"Public Health Laboratory, Newcastle General Hospital, Newcastle upon Tyne NE4 6BE"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.8.2359-2362.1998"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1997.2427"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2958.1993.tb01723.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.2.453-457.1998"},{"key":"e_1_3_2_6_2","first-page":"6","volume":"11","year":"2001","unstructured":"George, R. 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