{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T21:56:46Z","timestamp":1792619806740,"version":"4.5.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2002,4,1]],"date-time":"2002-04-01T00:00:00Z","timestamp":1017619200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,4]]},"abstract":"ABSTRACT<\/jats:title>\n \n Biofilm production has been implicated as a potential virulence factor of some\n Candida<\/jats:italic>\n species responsible for catheter-related fungemia in patients receiving parenteral nutrition. We therefore compared clinical bloodstream isolates representing seven different\n Candida<\/jats:italic>\n species to each other and to those from other anatomical sites for the capacity to form biofilms in glucose-containing medium. Potential associations between the capacity to form biofilms and the clinical characteristics of fungemia were also analyzed. Isolates included the following from nonneutropenic patients: 101 bloodstream isolates (35\n C. parapsilosis<\/jats:italic>\n , 30\n C. albicans<\/jats:italic>\n , 18\n C. tropicalis<\/jats:italic>\n , 8\n C. glabrata<\/jats:italic>\n , and 10 other\n Candida<\/jats:italic>\n species isolates) and 259 clinical isolates from other body sites (116\n C. albicans<\/jats:italic>\n , 53\n C. glabrata<\/jats:italic>\n , 43\n C. tropicalis<\/jats:italic>\n , 17\n C. parapsilosis<\/jats:italic>\n , and 30 other\n Candida<\/jats:italic>\n species isolates). Organisms were grown in Sabouraud dextrose broth (SDB) containing a final concentration of 8% glucose to induce biofilm formation, as published previously. Biofilm production was determined by both visual and spectrophotometric methods. In this medium, biofilm production by\n C. albicans<\/jats:italic>\n isolates was significantly less frequent (8%) than that by non-\n C. albicans Candida<\/jats:italic>\n species (61%;\n P<\/jats:italic>\n < 0.0001). The overall proportion of non-\n C. albicans Candida<\/jats:italic>\n species isolates from the blood that produced biofilms was significantly higher than that of non-\n C. albicans Candida<\/jats:italic>\n isolates obtained from other sites (79% versus 52%;\n P<\/jats:italic>\n = 0.0001). Bloodstream isolates of\n C. parapsilosis<\/jats:italic>\n alone were significantly more likely to be biofilm positive than were\n C. parapsilosis<\/jats:italic>\n isolates from other sites (86% versus 47%;\n P<\/jats:italic>\n = 0.0032). Non-\n C. albicans Candida<\/jats:italic>\n species, including\n C. parapsilosis<\/jats:italic>\n , were more likely to be biofilm positive if isolates were derived from patients whose candidemia was central venous catheter (CVC) related (95%;\n P<\/jats:italic>\n < 0.0001) and was associated with the use of total parenteral nutrition (TPN) (94%;\n P<\/jats:italic>\n < 0.005). These data suggest that the capacity of\n Candida<\/jats:italic>\n species isolates to produce biofilms in vitro in glucose-containing SDB may be a reflection of the pathogenic potential of these isolates to cause CVC-related fungemia in patients receiving TPN.\n <\/jats:p>","DOI":"10.1128\/jcm.40.4.1244-1248.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:59:40Z","timestamp":1027763980000},"page":"1244-1248","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":277,"title":["Biofilm Production by Isolates of\n Candida<\/i>\n Species Recovered from Nonneutropenic Patients: Comparison of Bloodstream Isolates with Isolates from Other Sources"],"prefix":"10.1128","volume":"40","author":[{"given":"Jong Hee","family":"Shin","sequence":"first","affiliation":[{"name":"Departments of Clinical Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seung Jung","family":"Kee","sequence":"additional","affiliation":[{"name":"Departments of Clinical Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myung Geun","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, Seonam University, College of Medicine, Gwangju, Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soo Hyun","family":"Kim","sequence":"additional","affiliation":[{"name":"Departments of Clinical Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong Hyeon","family":"Shin","sequence":"additional","affiliation":[{"name":"Internal Medicine, Chonnam National University Medical School"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sang Ku","family":"Lee","sequence":"additional","affiliation":[{"name":"Departments of Clinical Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Soon Pal","family":"Suh","sequence":"additional","affiliation":[{"name":"Departments of Clinical Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dong Wook","family":"Ryang","sequence":"additional","affiliation":[{"name":"Departments of Clinical Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/513663"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1086\/321811"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.2.452-456.1994"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00019048-199307000-00006"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.10.4221-4229.1992"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/22.Supplement_2.S73"},{"key":"e_1_3_2_8_2","first-page":"75","year":"1999","unstructured":"Evenson, M. A. 1999. Spectrophotometric techniques, p. 75-93. In C. A. Burtis and E. R. Ashwood (ed.), Tietz textbook of clinical chemistry, 3rd ed. W. B. Saunders Company, Philadelphia, Pa.","journal-title":"Tietz textbook of clinical chemistry"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1097\/00006454-199202000-00006"},{"key":"e_1_3_2_10_2","first-page":"265","year":"1995","unstructured":"Forbes, B. A., and P. A. Granato. 1995. Processing specimens for bacteria, p. 265-281. In P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (ed.), Manual of clinical microbiology, 6th ed. 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Parenteral nutrition, p. 1166-1170. In L. Goldman and J. C. Bennett (ed.), Cecil textbook of medicine, 21st ed. W. B. Saunders Company, Philadelphia, Pa.","journal-title":"Cecil textbook of medicine"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/14.Supplement_1.S120"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/22.Supplement_2.S89"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0732-8893(94)00114-C"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199411173312001"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.4.1258-1263.2001"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.6.1454-1459.1997"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1086\/321812"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.40.4.1244-1248.2002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.40.4.1244-1248.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T01:47:24Z","timestamp":1646444844000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.40.4.1244-1248.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,4]]},"references-count":23,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2002,4]]}},"alternative-id":["10.1128\/JCM.40.4.1244-1248.2002"],"URL":"https:\/\/doi.org\/10.1128\/jcm.40.4.1244-1248.2002","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,4]]},"assertion":[{"value":"2001-08-08","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-01-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}