{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,15]],"date-time":"2026-04-15T05:05:33Z","timestamp":1776229533620,"version":"3.50.1"},"reference-count":17,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2002,4,1]],"date-time":"2002-04-01T00:00:00Z","timestamp":1017619200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Identification of\n            <jats:italic>Nocardia<\/jats:italic>\n            to the species level is useful for predicting antimicrobial susceptibility patterns and defining the pathogenicity and geographic distribution of these organisms. We sought to develop an identification method which was accurate, timely, and employed tests which would be readily available in most clinical laboratories. We evaluated the API 20C AUX yeast identification system as well as several biochemical tests and Kirby-Bauer susceptibility patterns for the identification of 75 isolates encompassing the 8 medically relevant\n            <jats:italic>Nocardia<\/jats:italic>\n            species. There were few biochemical reactions that were sufficiently unique for species identification; of note,\n            <jats:italic>N. nova<\/jats:italic>\n            were positive for arylsulfatase,\n            <jats:italic>N. farcinica<\/jats:italic>\n            were positive for opacification of Middlebrook 7H11 agar, and\n            <jats:italic>N. brasiliensis<\/jats:italic>\n            and\n            <jats:italic>N. pseudobrasiliensis<\/jats:italic>\n            were the only species capable of liquefying gelatin. API 20C sugar assimilation patterns were unique for\n            <jats:italic>N. transvalensis<\/jats:italic>\n            ,\n            <jats:italic>N. asteroides<\/jats:italic>\n            IV, and\n            <jats:italic>N. brevicatena<\/jats:italic>\n            . There was overlap among the assimilation patterns for the other species. Species-specific patterns of susceptibility to gentamicin, tobramycin, amikacin, and erythromycin were obtained for\n            <jats:italic>N. nova<\/jats:italic>\n            ,\n            <jats:italic>N. farcinica<\/jats:italic>\n            , and\n            <jats:italic>N. brevicatena<\/jats:italic>\n            , while there was overlap among the susceptibility patterns for the other isolates. No single method could identify all\n            <jats:italic>Nocardia<\/jats:italic>\n            isolates to the species level; therefore, a combination of methods was necessary. An algorithm utilizing antibiotic susceptibility patterns, citrate utilization, acetamide utilization, and assimilation of inositol and adonitol accurately identified all isolates. The algorithm was expanded to include infrequent drug susceptibility patterns which have been reported in the literature but which were not seen in this study.\n          <\/jats:p>","DOI":"10.1128\/jcm.40.4.1346-1351.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:59:40Z","timestamp":1027763980000},"page":"1346-1351","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":97,"title":["Identification of Medically Relevant\n            <i>Nocardia<\/i>\n            Species with an Abbreviated Battery of Tests"],"prefix":"10.1128","volume":"40","author":[{"given":"Deanna L.","family":"Kiska","sequence":"first","affiliation":[{"name":"Department of Clinical Pathology, SUNY Upstate Medical University, Syracuse, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karen","family":"Hicks","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, SUNY Upstate Medical University, Syracuse, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David J.","family":"Pettit","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathology, SUNY Upstate Medical University, Syracuse, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.1.103-107.1996"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-2508(97)82456-4"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00442770"},{"key":"e_1_3_2_5_2","first-page":"370","year":"1999","unstructured":"Brown, J. M., M. M. McNeil, and E. P. Desmond. 1999. Nocardia, Rhodococcus, Gordona. Actinomadura, Streptomyces, and other actinomycetes of medical importance, p. 370-398. In P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (ed.), Manual of Clinical Microbiology, 7th ed. ASM Press, Washington, D.C.","journal-title":"Manual of Clinical Microbiology"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.1.158-164.2000"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.11.3040-3041.1993"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.8.2.127-133.1978"},{"key":"e_1_3_2_9_2","first-page":"340","year":"1991","unstructured":"Land, G., M. R. McGinnis, J. Staneck, and A. Gatson. 1991. Aerobic pathogenic Actinomycetales, p. 340-359. In A. Balows, W. J. Hausler, K. L. Herrmann, H. D. Isenberg, and H. J. Shadomy. (ed.), Manual of clinical microbiology, 5th ed. ASM Press, Washington, D.C.","journal-title":"Manual of clinical microbiology"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/22.6.891"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.7.3.357"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.12.3240-3243.1997"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.4.817-822.1997"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.6.1528-1533.1995"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.29.11.2407-2411.1991"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.12.2726-2732.1990"},{"key":"e_1_3_2_17_2","first-page":"1776","volume":"32","year":"1988","unstructured":"Wallace, R. J., Jr., L. C. Steele, G. Sumter, and J. M. Smith. 1988. Antimicrobial susceptibility patterns of Nocardia asteroides. J. Clin. Microbiol.32:1776-1779.","journal-title":"J. Clin. Microbiol."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.9.2235-2242.1997"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.40.4.1346-1351.2002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.40.4.1346-1351.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T01:48:37Z","timestamp":1646444917000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.40.4.1346-1351.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,4]]},"references-count":17,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2002,4]]}},"alternative-id":["10.1128\/JCM.40.4.1346-1351.2002"],"URL":"https:\/\/doi.org\/10.1128\/jcm.40.4.1346-1351.2002","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,4]]},"assertion":[{"value":"2001-08-31","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-02-02","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}