{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T11:55:19Z","timestamp":1789991719357,"version":"4.0.1"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2002,4,1]],"date-time":"2002-04-01T00:00:00Z","timestamp":1017619200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>Combined antigen and antibody screening (fourth-generation) assays reduce the diagnostic window period between the time of human immunodeficiency virus (HIV) infection and laboratory diagnosis by 4 days, on average, in comparison to antibody-only (third generation) enzyme immunoassays (EIAs). The aim of the present study was to assess whether the new VIDAS HIV DUO Ultra (Biome\u0301rieux, Marcy-l'Etoile, France) showed an improved sensitivity and specificity in comparison to licensed fourth-generation assays. A total of 16 seroconversion panels, 15 cell culture supernatants infected with different HIV type 1 (HIV-1) subtypes, and 257 potentially cross-reactive serum samples were tested with VIDAS DUO HIV Ultra, Genscreen Plus HIV Ag-Ab, Enzygnost HIV Integral, Enzymun-Test HIV Combi, Genscreen HIV 1\/2, version 2 (third-generation EIA), and Genetic Systems HIV-1 Ag EIA (p24 antigen assay). VIDAS HIV DUO Ultra showed a comparable sensitivity to the single p24 antigen assay in seroconversion panels and a dilution series of virus lysates. The diagnostic window was reduced with VIDAS HIV DUO Ultra by 3.82 days, on average, in comparison with the fourth-generation assay with the lowest sensitivity of the antigen detection module. HIV-1 infection was detected 5.88 days earlier than with third-generation EIA. The mean time delay between reverse transcription-PCR and VIDAS HIV DUO Ultra was only 2.31 days. The specificity of fourth-generation assays after retesting ranged between 98.1 and 100%. In conclusion, VIDAS HIV DUO Ultra can replace single-antigen screening for laboratory diagnosis and screening of HIV infection in blood donors. There was no evidence for a second diagnostic window due to impaired sensitivity of the antibody detection module of all the fourth-generation EIAs evaluated in the present study. The specificity after initial and\/or repeated testing of VIDAS HIV DUO Ultra was equivalent to that of a third-generation assay.<\/jats:p>","DOI":"10.1128\/jcm.40.4.1420-1426.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T09:59:40Z","timestamp":1027763980000},"page":"1420-1426","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":53,"title":["Evaluation of a New Combined Antigen and Antibody Human Immunodeficiency Virus Screening Assay, VIDAS HIV DUO Ultra"],"prefix":"10.1128","volume":"40","author":[{"given":"Bernard","family":"Weber","sequence":"first","affiliation":[{"name":"Laboratoires Re\u0301unis Kutter-Lieners-Hastert, Junglinster, Luxembourg"},{"name":"Institut fu\u0308r Medizinische Virologie, Universita\u0308tskliniken Frankfurt, Frankfurt, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annemarie","family":"Berger","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Medizinische Virologie, Universita\u0308tskliniken Frankfurt, Frankfurt, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Holger","family":"Rabenau","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Medizinische Virologie, Universita\u0308tskliniken Frankfurt, Frankfurt, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans Wilhelm","family":"Doerr","sequence":"additional","affiliation":[{"name":"Institut fu\u0308r Medizinische Virologie, Universita\u0308tskliniken Frankfurt, Frankfurt, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199011083231905"},{"key":"e_1_3_2_3_2","first-page":"45","volume":"45","year":"1997","unstructured":"Aubuchon, J. P., J. D. Birkmeyer, and M. P. Busch. 1997. Cost-effectiveness of expanded human immunodeficiency virus-testing protocols for donated blood. Transfusion45:45-51.","journal-title":"Transfusion"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.11.2846-2853.1997"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(00)00229-9"},{"key":"e_1_3_2_6_2","unstructured":"Nicht-parametrierbare Methoden 1978"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.2000.40020143.x"},{"key":"e_1_3_2_8_2","first-page":"1","volume":"38","year":"1989","unstructured":"Centers for Disease Control. 1989. Interpretation and use of the Western blot assay for serodiagnosis of human immunodeficiency virus type 1 infections. Morb. Mortal. Wkly. Rep.38:1-7.","journal-title":"Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_2_9_2","first-page":"4","volume":"155","year":"1999","unstructured":"Courouc\u00e9, A. M., et al. 1999. Tests de d\u00e9pistage combin\u00e9 des anticorps anti-VIH et de l'antig\u00e8ne p24. Gaz. Transfus. Sang.155:4-18.","journal-title":"Gaz. Transfus. Sang."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-199212000-00024"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198910053211405"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(98)00094-9"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.3.1581-1585.1994"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1246-7820(00)80011-7"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1999.39101141.x"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.6.2459-2461.2000"},{"key":"e_1_3_2_17_2","first-page":"104","volume":"20","year":"2001","unstructured":"Ly, T. D., S. Laperche, and A. M. Courouc\u00e9. 2001. Early detection of human immunodeficiency virus infection using third- and fourth-generation screening assays. Eur. J. Clin. Microbiol. Infect. Dis.20:104-110.","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.9.3122-3128.2001"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1089\/aid.1993.9.997"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1537-2995.1999.39070688.x"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)06318-1"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.7.2518-2524.2001"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(89)90521-7"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(99)00086-5"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(98)00126-8"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.8.2235-2239.1998"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-0934(98)00162-1"},{"key":"e_1_3_2_28_2","first-page":"319","volume":"27","year":"1999","unstructured":"Yerly, S., F. Simon, and L. Perrin. 1999. Early diagnosis of primary HIV infections: using a combined screening test (p24 antigen and anti-HIV antibodies). Schweiz. Med. Wochenschr.27:319-322.","journal-title":"Schweiz. Med. Wochenschr."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.40.4.1420-1426.2002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.40.4.1420-1426.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T01:47:59Z","timestamp":1646444879000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.40.4.1420-1426.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,4]]},"references-count":27,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2002,4]]}},"alternative-id":["10.1128\/JCM.40.4.1420-1426.2002"],"URL":"https:\/\/doi.org\/10.1128\/jcm.40.4.1420-1426.2002","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,4]]},"assertion":[{"value":"2001-08-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-01-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}