{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,29]],"date-time":"2026-10-29T22:58:40Z","timestamp":1793314720470,"version":"4.5.0"},"reference-count":40,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2002,5,1]],"date-time":"2002-05-01T00:00:00Z","timestamp":1020211200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The bacteria found in carious dentine were correlated with the tissue response of the dental pulps of 65 teeth extracted from patients with advanced caries and pulpitis. Standardized homogenates of carious dentine were plated onto selective and nonselective media under anaerobic and microaerophilic conditions. In addition, real-time PCR was used to quantify the recovery of anaerobic bacteria. Primers and fluorogenic probes were designed to detect the total anaerobic microbial load, the genera\n            <jats:italic>Prevotella<\/jats:italic>\n            and\n            <jats:italic>Fusobacterium<\/jats:italic>\n            , and the species\n            <jats:italic>Prevotella melaninogenica<\/jats:italic>\n            ,\n            <jats:italic>Porphyromonas endodontalis<\/jats:italic>\n            ,\n            <jats:italic>Porphyromonas gingivalis<\/jats:italic>\n            , and\n            <jats:italic>Micromonas<\/jats:italic>\n            (formerly\n            <jats:italic>Peptostreptococcus<\/jats:italic>\n            )\n            <jats:italic>micros<\/jats:italic>\n            . The pulpal pathology was categorized according to the cellular response and degenerative changes. Analysis of cultured bacteria showed a predominance of gram-positive microorganisms, particularly lactobacilli. Gram-negative bacteria were also present in significant numbers with\n            <jats:italic>Prevotella<\/jats:italic>\n            spp., the most numerous anaerobic group cultured. Real-time PCR analysis indicated a greater microbial load than that determined by colony counting. The total number of anaerobes detected was 41-fold greater by real-time PCR than by colony counting, while the numbers of\n            <jats:italic>Prevotella<\/jats:italic>\n            and\n            <jats:italic>Fusobacterium<\/jats:italic>\n            spp. detected were 82- and 2.4-fold greater by real-time PCR than by colony counting, respectively. Real-time PCR also identified\n            <jats:italic>M. micros<\/jats:italic>\n            ,\n            <jats:italic>P. endodontalis<\/jats:italic>\n            , and\n            <jats:italic>P. gingivalis<\/jats:italic>\n            in 71, 60, and 52% of carious samples, respectively. Correlation matrices of the real-time PCR data revealed significant positive associations between\n            <jats:italic>M. micros<\/jats:italic>\n            and\n            <jats:italic>P. endodontalis<\/jats:italic>\n            detection and inflammatory degeneration of pulpal tissues. These anaerobes have been strongly implicated in endodontic infections that occur as sequelae to carious pulpitis. Accordingly, the data suggest that the presence of high levels of these bacteria in carious lesions may be indicative of irreversible pulpal pathology.\n          <\/jats:p>","DOI":"10.1128\/jcm.40.5.1698-1704.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:09:29Z","timestamp":1027764569000},"page":"1698-1704","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":239,"title":["Quantitative Microbiological Study of Human Carious Dentine by Culture and Real-Time PCR: Association of Anaerobes with Histopathological Changes in Chronic Pulpitis"],"prefix":"10.1128","volume":"40","author":[{"given":"F. Elizabeth","family":"Martin","sequence":"first","affiliation":[{"name":"Institute of Dental Research, Westmead Centre for Oral Health, Westmead Hospital, Westmead, NSW, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mangala A.","family":"Nadkarni","sequence":"additional","affiliation":[{"name":"Institute of Dental Research, Westmead Centre for Oral Health, Westmead Hospital, Westmead, NSW, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas A.","family":"Jacques","sequence":"additional","affiliation":[{"name":"Institute of Dental Research, Westmead Centre for Oral Health, Westmead Hospital, Westmead, NSW, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil","family":"Hunter","sequence":"additional","affiliation":[{"name":"Institute of Dental Research, Westmead Centre for Oral Health, Westmead Hospital, Westmead, NSW, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1902\/jop.1994.65.11.1046"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1992.tb00035.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.2.244-269.2001"},{"key":"e_1_3_2_6_2","unstructured":"Br\u00e4nnstr\u00f6mm M. 1981. 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