{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T13:59:03Z","timestamp":1791035943082,"version":"4.1.0"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2002,6,1]],"date-time":"2002-06-01T00:00:00Z","timestamp":1022889600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>The study of 16S rRNA gene sequences of all isolates of<jats:italic>Bartonella henselae<\/jats:italic>obtained in our laboratory and others from human patients or cats has revealed two genotypes according to the sequence of the 16S rRNA gene. Two isolates of these genotypes have previously been related to two different serotypes, and lack of cross-protection of the two serotypes has been demonstrated in cats. We investigated the grouping of eight strains of<jats:italic>B. henselae<\/jats:italic>on the basis of 16S ribosomal DNA, 35-kDa protein, Pap 31 protein, and internal transcribed spacer (ITS) gene sequencing; sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) profiles; and monoclonal antibody reactivity studies. Houston-1, 90-615, and SA2 strains showed the same patterns in SDS-PAGE, but they differed from the patterns of<jats:italic>B. henselae<\/jats:italic>isolates URBHLLY8, URBHLIE9, Cat6, Fizz, and CAL-1. Nine monoclonal antibodies derived from BALB\/c mice immunized with<jats:italic>B. henselae<\/jats:italic>Houston-1 strain reacted only with strains Houston-1, 90-615, and SA2, and not with any other<jats:italic>Bartonella<\/jats:italic>strains. The two serogroups corresponded with two genotypes based on differences in the sequences of the genes encoding 16S rRNA, 35-kDa protein, and Pap 31 protein. Sequences of ITS genes were highly divergent among strains, as each had a unique sequence and the subdivision was not supported by DNA-DNA relatedness study. Study of 22 additional strains of<jats:italic>B. henselae<\/jats:italic>isolated from French bacteremic cats demonstrated that they all belong to one or the other of the proposed serotype or genotype.<\/jats:p>","DOI":"10.1128\/jcm.40.6.2002-2008.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:09:29Z","timestamp":1027764569000},"page":"2002-2008","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":50,"title":["Genotypic Characteristics of Two Serotypes of<i>Bartonella henselae<\/i>"],"prefix":"10.1128","volume":"40","author":[{"given":"Bernard","family":"La Scola","sequence":"first","affiliation":[{"name":"Unite\u0301 des Rickettsies, CNRS UMR 6020, 13385 Marseille Cedex 5"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongxing","family":"Liang","sequence":"additional","affiliation":[{"name":"Unite\u0301 des Rickettsies, CNRS UMR 6020, 13385 Marseille Cedex 5"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zaher","family":"Zeaiter","sequence":"additional","affiliation":[{"name":"Unite\u0301 des Rickettsies, CNRS UMR 6020, 13385 Marseille Cedex 5"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Houpikian","sequence":"additional","affiliation":[{"name":"Unite\u0301 des Rickettsies, CNRS UMR 6020, 13385 Marseille Cedex 5"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick A. D.","family":"Grimont","sequence":"additional","affiliation":[{"name":"Unite\u0301 des Ente\u0301robacte\u0301ries, Unite\u0301 INSERM 199, Institut Pasteur, 75724 Paris Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Raoult","sequence":"additional","affiliation":[{"name":"Unite\u0301 des Rickettsies, CNRS UMR 6020, 13385 Marseille Cedex 5"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.10.3159-3166.1999"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.2.254-260.1996"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-45-1-1"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1099\/00207713-46-4-891"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0378-1119(97)00580-5"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.109.3.953-965.1972"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199901213400303"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.4.872-881.1993"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-118-5-199303010-00002"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(96)90012-4"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.5.1158-1160.1996"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.4.1117-1122.1999"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-200107000-00003"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-199814000-00011"},{"key":"e_1_3_2_16_2","first-page":"287","volume":"17","year":"1993","unstructured":"Gradon, J. D., and D. S. Stein. 1993. Association between Rochalimaea infection and cat-scratch disease. Clin. Infect. Dis.17:287-288.","journal-title":"Clin. Infect. Dis."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02605371"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.8.2120-2123.1997"},{"key":"e_1_3_2_19_2","first-page":"139","year":"1988","unstructured":"Harlow, E., and D. Lane. 1988. Monoclonal antibodies, growing hybridomas, p. 139-282. In E. Harlow and D. Lane (ed.), Antibodies: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.","journal-title":"Antibodies: a laboratory manual"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.6.1327-1331.1997"},{"key":"e_1_3_2_21_2","first-page":"679","volume":"140","year":"1989","unstructured":"Khammas, K. M., E. Ageron, P. A. D. Grimont, and P. Kaiser. 1989. Azospirillum irakense sp. nov. a nitrogen-fixing bacterium associated with rice roots and rhizosphere soil. Res. Microbiol.140:679-693.","journal-title":"Res. Microbiol."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1994.03510310061039"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_3_2_24_2","unstructured":"La Scola B. B. Davoust M. Boni and D. Raoult. Lack of correlation between serology Bartonella DNA detection within fleas and results of blood culture in a Bartonella infected stray cat population. Clin. Microbiol. Infect. in press."},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.6.1899-1905.1999"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.7.4.617-624.2000"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.7.1730-1734.1993"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01708232"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.7.1797-1803.1995"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199211263272215"},{"key":"e_1_3_2_31_2","doi-asserted-by":"crossref","first-page":"1961","DOI":"10.4049\/jimmunol.121.5.1961","volume":"121","year":"1978","unstructured":"Philip, R. N., E. A. Casper, W. Burgdorfer, R. K. Gerloff, L. E. Hugues, and E. J. Bell. 1978. Serologic typing of rickettsiae of the spotted fever group by micro immunofluorescence. J. Immunol.121:1961-1968.","journal-title":"J. Immunol."},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-125-8-199610150-00004"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.2.265-274.1992"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199012063232301"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.5.1089-1093.1995"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.6.1573-1579.1995"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(94)00919-J"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.3.584-587.1997"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1080\/00365549850160693"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199012063232303"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.1992.00400150114021"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/80.5.714"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1969.18.708"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.173.2.697-703.1991"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.19.3.1013-1020.1978"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.2.275-280.1992"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(98)00154-8"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.3.1023-1030.2002"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.40.6.2002-2008.2002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.40.6.2002-2008.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,4,23]],"date-time":"2023-04-23T16:05:24Z","timestamp":1682265924000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.40.6.2002-2008.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,6]]},"references-count":47,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2002,6]]}},"alternative-id":["10.1128\/JCM.40.6.2002-2008.2002"],"URL":"https:\/\/doi.org\/10.1128\/jcm.40.6.2002-2008.2002","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,6]]},"assertion":[{"value":"2001-05-29","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-02-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}