{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,30]],"date-time":"2025-10-30T06:47:41Z","timestamp":1761806861821},"reference-count":13,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2002,6,1]],"date-time":"2002-06-01T00:00:00Z","timestamp":1022889600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Aspergilloma and invasive aspergillosis are important opportunistic infections caused by\n            <jats:italic>Aspergillus<\/jats:italic>\n            species, among which\n            <jats:italic>Aspergillus fumigatus<\/jats:italic>\n            is the most common species associated with human disease. We developed an enzyme-linked immunosorbent assay (ELISA)-based antibody assay with Afmp1p, a purified recombinant antigenic cell wall galactomannoprotein of\n            <jats:italic>A. fumigatus<\/jats:italic>\n            . Evaluation of the test with guinea pig sera against\n            <jats:italic>A. fumigatus<\/jats:italic>\n            and other pathogenic fungi indicated that this assay was specific for\n            <jats:italic>A. fumigatus<\/jats:italic>\n            . Clinical evaluation revealed that the assay was 100% sensitive for patients with aspergilloma and 33.3% sensitive for patients with invasive aspergillosis. No false-positive results were found for serum samples from 80 healthy blood donors, 6 patients with typhoid fever, 4 patients with melioidosis, 20 patients with penicilliosis marneffei, 5 patients with candidiasis, and 4 patients with cryptococcosis, indicating a high specificity of the test. Thus, this ELISA-based test for the detection of anti-Afmp1p antibody can be of significant value as a diagnostic for aspergillosis.\n          <\/jats:p>","DOI":"10.1128\/jcm.40.6.2041-2045.2002","type":"journal-article","created":{"date-parts":[[2002,7,27]],"date-time":"2002-07-27T10:09:29Z","timestamp":1027764569000},"page":"2041-2045","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":55,"title":["Detection of Antibodies Specific to an Antigenic Cell Wall Galactomannoprotein for Serodiagnosis of\n            <i>Aspergillus fumigatus<\/i>\n            Aspergillosis"],"prefix":"10.1128","volume":"40","author":[{"given":"Che-man","family":"Chan","sequence":"first","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, Queen Mary Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick C. Y.","family":"Woo","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, Queen Mary Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andy S. P.","family":"Leung","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, Queen Mary Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susanna K. P.","family":"Lau","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, Queen Mary Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiao-yan","family":"Che","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, Queen Mary Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, Queen Mary Hospital"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kwok-yung","family":"Yuen","sequence":"additional","affiliation":[{"name":"Department of Microbiology, The University of Hong Kong, Queen Mary Hospital"},{"name":"HKU-Pasteur Research Centre, Hong Kong"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.66.3.966-973.1998"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.4.981-986.1999"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.10.3028-3031.1998"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/12.6.1147"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.2.558-562.2000"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.12.2.310"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.17.4.708-710.1983"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02013657"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.5.1721-1730.2001"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.12.4535-4540.2001"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.12.4598-4600.2001"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.11.3830-3837.2001"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1086\/514492"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.40.6.2041-2045.2002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.40.6.2041-2045.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T01:51:30Z","timestamp":1646445090000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.40.6.2041-2045.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,6]]},"references-count":13,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2002,6]]}},"alternative-id":["10.1128\/JCM.40.6.2041-2045.2002"],"URL":"https:\/\/doi.org\/10.1128\/jcm.40.6.2041-2045.2002","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,6]]},"assertion":[{"value":"2001-12-14","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-03-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}