{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T12:49:06Z","timestamp":1791118146411,"version":"4.1.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2002,7,1]],"date-time":"2002-07-01T00:00:00Z","timestamp":1025481600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Cryptosporidiosis is a significant cause of food-borne and waterborne outbreaks of diarrheal diseases. To better understand the route of transmission of\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            parasites, a number of genotyping techniques have been developed, based on PCR-restriction fragment length polymorphism or sequencing analysis of antigen, structural, and housekeeping genes. In this study, a real-time assay for the detection of\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            oocysts is described. This technique had a detection limit of five oocysts. By melting curve analysis of PCR products with fluorescence-labeled hybridization probes, this technique was able to differentiate five common\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            parasites that are pathogenic for humans in a single PCR. We evaluated and validated the test using samples from presently known\n            <jats:italic>Cryptosporidium<\/jats:italic>\n            parasites that are pathogenic for humans. This technique provides an alternative molecular tool in epidemiologic studies of human cryptosporidiosis.\n          <\/jats:p>","DOI":"10.1128\/jcm.40.7.2335-2338.2002","type":"journal-article","created":{"date-parts":[[2002,7,28]],"date-time":"2002-07-28T23:07:23Z","timestamp":1027897643000},"page":"2335-2338","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":72,"title":["Detection and Differentiation of\n            <i>Cryptosporidium<\/i>\n            Parasites That Are Pathogenic for Humans by Real-Time PCR"],"prefix":"10.1128","volume":"40","author":[{"given":"Josef R.","family":"Limor","sequence":"first","affiliation":[{"name":"Division of Parasitic Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30341"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Altaf 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