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The assay included an automated DNA extraction protocol conducted with a MagNA Pure instrument and real-time PCR conducted with a LightCycler instrument. The performance and robustness of the assay were evaluated for a suspension of methicillin-resistant<jats:italic>S. aureus<\/jats:italic>(MRSA) strain with a turbidity equivalent to a McFarland standard of 0.5, which was found to be the ideal working concentration. The specificity of the new molecular assay was tested with a panel of 30 gram-negative and gram-positive bacterial strains other than MRSA. No cross-reactivity was observed. In a clinical study, 109 isolates of MRSA were investigated. All clinical MRSA isolates gave positive results for the<jats:italic>S. aureus<\/jats:italic>-specific genomic target, and all but one were positive for the<jats:italic>mecA<\/jats:italic>gene. In conclusion, the new molecular assay was found to be quick, robust, and laborsaving, and it proved to be suitable for a routine molecular diagnostic laboratory.<\/jats:p>","DOI":"10.1128\/jcm.40.7.2392-2397.2002","type":"journal-article","created":{"date-parts":[[2002,7,28]],"date-time":"2002-07-28T23:07:23Z","timestamp":1027897643000},"page":"2392-2397","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":72,"title":["Detection of Methicillin-Resistant<i>Staphylococcus aureus<\/i>and Simultaneous Confirmation by Automated Nucleic Acid Extraction and Real-Time PCR"],"prefix":"10.1128","volume":"40","author":[{"given":"Andrea J.","family":"Grisold","sequence":"first","affiliation":[{"name":"Institute of Hygiene, Karl Franzens University Graz, A-8010 Graz"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Leitner","sequence":"additional","affiliation":[{"name":"Institute of Hygiene, Karl Franzens University Graz, A-8010 Graz"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gerhard","family":"Mu\u0308hlbauer","sequence":"additional","affiliation":[{"name":"Roche Diagnostics GmbH, A-1211 Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Egon","family":"Marth","sequence":"additional","affiliation":[{"name":"Institute of Hygiene, Karl Franzens University Graz, A-8010 Graz"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald H.","family":"Kessler","sequence":"additional","affiliation":[{"name":"Institute of Hygiene, Karl Franzens University Graz, A-8010 Graz"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1006\/mcpr.1996.0066"},{"key":"e_1_3_2_3_2","first-page":"741","year":"1968","unstructured":"Benner, E. 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