{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T13:46:48Z","timestamp":1774532808514,"version":"3.50.1"},"reference-count":8,"publisher":"American Society for Microbiology","issue":"7","license":[{"start":{"date-parts":[[2002,7,1]],"date-time":"2002-07-01T00:00:00Z","timestamp":1025481600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            One hundred BacT\/ALERT blood culture bottles growing gram-positive cocci in clusters were cultured and studied by LightCycler PCR for the\n            <jats:italic>sa442<\/jats:italic>\n            and\n            <jats:italic>mecA<\/jats:italic>\n            genes. PCR was 100% sensitive and specific for detecting\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            and methicillin resistance in\n            <jats:italic>S. aureus<\/jats:italic>\n            but was less accurate for methicillin resistance in coagulase-negative staphylococci.\n          <\/jats:p>","DOI":"10.1128\/jcm.40.7.2659-2661.2002","type":"journal-article","created":{"date-parts":[[2002,7,28]],"date-time":"2002-07-28T23:07:23Z","timestamp":1027897643000},"page":"2659-2661","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":101,"title":["Rapid Identification of\n            <i>Staphylococcus aureus<\/i>\n            and the\n            <i>mecA<\/i>\n            Gene from BacT\/ALERT Blood Culture Bottles by Using the LightCycler System"],"prefix":"10.1128","volume":"40","author":[{"given":"Nabin K.","family":"Shrestha","sequence":"first","affiliation":[{"name":"Section of Clinical Microbiology"},{"name":"Division of Infectious Diseases, The Cleveland Clinic Foundation, Cleveland, Ohio"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marion J.","family":"Tuohy","sequence":"additional","affiliation":[{"name":"Section of Clinical Microbiology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerri S.","family":"Hall","sequence":"additional","affiliation":[{"name":"Section of Clinical Microbiology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carlos M.","family":"Isada","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, The Cleveland Clinic Foundation, Cleveland, Ohio"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary W.","family":"Procop","sequence":"additional","affiliation":[{"name":"Section of Clinical Microbiology"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.8.2961-2963.2001"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(01)00301-7"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.3.618-623.1998"},{"key":"e_1_3_1_5_2","unstructured":"NCCLS. 2001. Performance standards for antimicrobial susceptibility testing. Eleventh Informational Supplement. M100-S11. 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