{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T10:58:02Z","timestamp":1790506682138,"version":"4.1.0"},"reference-count":43,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2002,8,1]],"date-time":"2002-08-01T00:00:00Z","timestamp":1028160000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The\n                    <jats:italic>Echinococcus multilocularis<\/jats:italic>\n                    protein Em18 is one of the most promising antigens for use in serodiagnosis of alveolar echinococcosis in human patients. Here we identify an antigenic relationship between Em18 and a 65-kDa immunodominant\n                    <jats:italic>E. multilocularis<\/jats:italic>\n                    surface protein previously identified as either EM10 or EmII\/3. The NH\n                    <jats:sub>2<\/jats:sub>\n                    -terminal sequence of native Em18 was determined, revealing it to be a fragment of EM10. Experiments were undertaken to investigate the effect of proteinase inhibitors on the degradation of EM10 in crude extracts of\n                    <jats:italic>E. multilocularis<\/jats:italic>\n                    protoscoleces. Em18 was found to be the product of degradation of EM10 by cysteine proteinase. A recombinant Em18 (RecEm18, derived from\n                    <jats:sup>349<\/jats:sup>\n                    K to\n                    <jats:sup>508<\/jats:sup>\n                    K of EM10) was successfully expressed by using\n                    <jats:italic>Escherichia coli<\/jats:italic>\n                    expression system and then evaluated for use in serodiagnosis of alveolar echinococcosis. RecEm18 was recognized by 27 (87.1%) and 28 (90.3%) of 31 serum samples from clinically and\/or pathologically confirmed alveolar echinococcosis patients by enzyme-linked immunosorbent assay and immunoblotting, respectively. Of 33 serum samples from cystic echinococcosis patients, 1 was recorded as having a weak positive reaction to RecEm18; however, none of the serum samples which were tested from neurocysticercosis patients (\n                    <jats:italic>n<\/jats:italic>\n                    = 10) or healthy people (\n                    <jats:italic>n<\/jats:italic>\n                    = 15) showed positive reactions. RecEm18 has the potential for use in the differential serodiagnosis of alveolar echinococcosis.\n                  <\/jats:p>","DOI":"10.1128\/jcm.40.8.2760-2765.2002","type":"journal-article","created":{"date-parts":[[2002,10,1]],"date-time":"2002-10-01T15:36:19Z","timestamp":1033486579000},"page":"2760-2765","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":67,"title":["Alveolar Echinococcosis: Characterization of Diagnostic Antigen Em18 and Serological Evaluation of Recombinant Em18"],"prefix":"10.1128","volume":"40","author":[{"given":"Yasuhito","family":"Sako","sequence":"first","affiliation":[{"name":"Department of Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minoru","family":"Nakao","sequence":"additional","affiliation":[{"name":"Department of Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuhiro","family":"Nakaya","sequence":"additional","affiliation":[{"name":"Animal Laboratory for Medical Research, Asahikawa Medical College, Asahikawa, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Yamasaki","sequence":"additional","affiliation":[{"name":"Department of Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Gottstein","sequence":"additional","affiliation":[{"name":"Institute of Parasitology, University of Berne, Berne, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marshall W.","family":"Lightowers","sequence":"additional","affiliation":[{"name":"Veterinary Clinical Center, University of Melbourne, Werribee, Melbourne, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter M.","family":"Schantz","sequence":"additional","affiliation":[{"name":"Division of Parasitic Diseases, NCID, Centers for Disease Control and Prevention, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akira","family":"Ito","sequence":"additional","affiliation":[{"name":"Department of Parasitology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1990.43.506"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.107.9.2509"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000079300"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000076344"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(91)90108-I"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00932958"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.6.8.1061"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3024.1985.tb00070.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(87)90365-8"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.12.3211-3215.1993"},{"key":"e_1_3_2_12_2","first-page":"56","volume":"44","year":"1991","unstructured":"Hemmings, L., and D. 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