{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T08:19:01Z","timestamp":1788682741040,"version":"build-2803163510"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2002,9,1]],"date-time":"2002-09-01T00:00:00Z","timestamp":1030838400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Ten nonrepetitive clinical isolates of\n            <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n            exhibiting an unusual inducible \u03b2-lactam resistance phenotype were identified between January 1999 and September 2001 in a university hospital in Taiwan. In the presence of 2 \u03bcg of clavulanic acid, the isolates showed a one to four twofold concentration increase in the MICs of ceftazidime, cefotaxime, and aztreonam but remained susceptible to cefepime (MICs, \u22640.5 \u03bcg\/ml) and imipenem (MICs, \u22640.5 \u03bcg\/ml). PCR, sequence analysis, and isoelectric focusing revealed production by these isolates of TEM-1, SHV-11, and DHA-1, a plasmid-encoded inducible AmpC \u03b2-lactamase originally found in a\n            <jats:italic>Salmonella enterica<\/jats:italic>\n            serovar Enteritidis strain. Transfer of the resistance by conjugation experiments was not successful, but Southern hybridization showed that\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>DHA-1<\/jats:sub>\n            was located on 70-kb plasmids, suggesting that the\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>DHA-1<\/jats:sub>\n            -containing plasmids in the\n            <jats:italic>K. pneumoniae<\/jats:italic>\n            isolates were non-self-transmissible. Five isolates were recovered from patients in two surgery wards and two intensive care units. Acquisition of the DHA-1 producers could be traced back to previous hospitalizations 1 to 5 months earlier for the other five patients. Six and seven patterns among the isolates were demonstrated by plasmid analysis and ribotyping, respectively, indicating that the spread of the DHA-1 producers was due to both horizontal transfer of\n            <jats:italic>bla<\/jats:italic>\n            <jats:sub>DHA-1<\/jats:sub>\n            and dissemination of endemic clones.\n          <\/jats:p>","DOI":"10.1128\/jcm.40.9.3121-3126.2002","type":"journal-article","created":{"date-parts":[[2002,8,29]],"date-time":"2002-08-29T19:27:50Z","timestamp":1030649270000},"page":"3121-3126","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":60,"title":["Emergence of\n            <i>Klebsiella pneumoniae<\/i>\n            Isolates Producing Inducible DHA-1 \u03b2-Lactamase in a University Hospital in Taiwan"],"prefix":"10.1128","volume":"40","author":[{"given":"Jing-Jou","family":"Yan","sequence":"first","affiliation":[{"name":"Departments of Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen-Chien","family":"Ko","sequence":"additional","affiliation":[{"name":"Internal Medicine"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-Chih","family":"Jung","sequence":"additional","affiliation":[{"name":"Department of Pathology, Sinlau Christian Hospital, Tainan, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chin-Luan","family":"Chuang","sequence":"additional","affiliation":[{"name":"Departments of Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiunn-Jong","family":"Wu","sequence":"additional","affiliation":[{"name":"Medical Technology, College of Medicine, National Cheng Kung University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"1451","volume":"44","year":"1980","unstructured":"Bachmann, B. 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M. 1995. \u03b2-Lactamases in laboratory and clinical resistance. Clin. Microbiol. Rev.34:557-584.","journal-title":"Clin. Microbiol. Rev."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/48.suppl_1.59"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-88-1-169"},{"key":"e_1_3_2_19_2","unstructured":"National Committee for Clinical Laboratory Standards. 2000. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically 5th ed. Approved standard M7-A5. National Committee for Clinical Laboratory Standards Wayne Pa."},{"key":"e_1_3_2_20_2","unstructured":"National Committee for Clinical Laboratory Standards. 2000. Performance standards for antimicrobial disk susceptibility tests 7th ed. Approved standard M2-A7. 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