{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T16:01:01Z","timestamp":1792080061963,"version":"4.4.0"},"reference-count":17,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2002,9,1]],"date-time":"2002-09-01T00:00:00Z","timestamp":1030838400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>An automated ribotyping device (RiboPrinter) was used to determine the ribotypes of a collection of<jats:italic>Burkholderia pseudomallei<\/jats:italic>isolates. In a preliminary evaluation with the restriction enzymes<jats:italic>Bam<\/jats:italic>HI and<jats:italic>Eco<\/jats:italic>RI, the protocol with<jats:italic>Eco<\/jats:italic>RI was more discriminating. The reproducibilities of the ribotypes obtained with<jats:italic>Eco<\/jats:italic>RI (<jats:italic>Eco<\/jats:italic>RI ribotypes) were determined by testing three levels of bacterial loads. The performance of the manufacturer's software was assessed by comparing the machine-optimized ribotypes with the type determined from the original gel image analyzed with Bionumerics software. The library of<jats:italic>B. pseudomallei Eco<\/jats:italic>RI ribotypes was then compared with the ribotypes obtained by DNA macrorestriction analysis of<jats:italic>Xba<\/jats:italic>I digests by pulsed-field gel electrophoresis. The typeability of<jats:italic>B. pseudomallei<\/jats:italic>by<jats:italic>Eco<\/jats:italic>RI ribotyping was 100%, and the discrimination index was 0.94. The slightly greater discrimination provided by DNA macrorestriction analysis (0.96) was achieved at the expense of a significantly longer processing time of 6 days, although the method was only half the cost of automated ribotyping. Typeability by macrorestriction analysis was lower (97%) unless a thiourea step was added to neutralize the action of Tris-dependent endonucleases. The digital record of<jats:italic>B. pseudomallei<\/jats:italic>isolates analyzed thus far provides a useful resource for future epidemiological studies and will help shorten the response time in the event of a further melioidosis outbreak or the deliberate release of<jats:italic>B. pseudomallei<\/jats:italic>as a biohazard.<\/jats:p>","DOI":"10.1128\/jcm.40.9.3198-3203.2002","type":"journal-article","created":{"date-parts":[[2002,8,29]],"date-time":"2002-08-29T19:27:50Z","timestamp":1030649270000},"page":"3198-3203","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Comparison of Rapid, Automated Ribotyping and DNA Macrorestriction Analysis of<i>Burkholderia pseudomallei<\/i>"],"prefix":"10.1128","volume":"40","author":[{"given":"Timothy J. 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