{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T10:39:05Z","timestamp":1791196745455,"version":"4.1.0"},"reference-count":39,"publisher":"American Society for Microbiology","issue":"9","license":[{"start":{"date-parts":[[2002,9,1]],"date-time":"2002-09-01T00:00:00Z","timestamp":1030838400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2002,9]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Several real-time PCR procedures for the detection and genotyping of oocysts of\n            <jats:italic>Cryptosporidium parvum<\/jats:italic>\n            were evaluated. A 40-cycle amplification of a 157-bp fragment from the\n            <jats:italic>C. parvum<\/jats:italic>\n            \u03b2-tubulin gene detected individual oocysts which were introduced into the reaction mixture by micromanipulation. SYBR Green I melting curve analysis was used to confirm the specificity of the method when DNA extracted from fecal samples spiked with oocysts was analyzed. Because\n            <jats:italic>C. parvum<\/jats:italic>\n            isolates infecting humans comprise two distinct genotypes, designated type 1 and type 2, real-time PCR methods for discriminating\n            <jats:italic>C. parvum<\/jats:italic>\n            genotypes were developed. The first method used the same \u03b2-tubulin amplification primers and two fluorescently labeled antisense oligonucleotide probes spanning a 49-bp polymorphic sequence diagnostic for\n            <jats:italic>C. parvum<\/jats:italic>\n            type 1 and type 2. The second genotyping method used SYBR Green I fluorescence and targeted a polymorphic coding region within the GP900\/poly(T) gene. Both methods discriminated between type 1 and type 2\n            <jats:italic>C. parvum<\/jats:italic>\n            on the basis of melting curve analysis. To our knowledge, this is the first report describing the application of melting curve analysis for genotyping of\n            <jats:italic>C. parvum<\/jats:italic>\n            oocysts.\n          <\/jats:p>","DOI":"10.1128\/jcm.40.9.3237-3244.2002","type":"journal-article","created":{"date-parts":[[2002,8,29]],"date-time":"2002-08-29T19:27:50Z","timestamp":1030649270000},"page":"3237-3244","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":68,"title":["Detection and Genotyping of Oocysts of\n            <i>Cryptosporidium parvum<\/i>\n            by Real-Time PCR and Melting Curve Analysis"],"prefix":"10.1128","volume":"40","author":[{"given":"Sultan","family":"Tanr\u0131verdi","sequence":"first","affiliation":[{"name":"Department of Pediatric Hematology-Oncology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atila","family":"Tanyeli","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology-Oncology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fikri","family":"Bas\u0327lam\u0131s\u0327l\u0131","sequence":"additional","affiliation":[{"name":"Department of Hematology-Oncology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatih","family":"Ko\u0308ksal","sequence":"additional","affiliation":[{"name":"Department of Microbiology, School of Medicine, C\u0327ukurova University, 01330 Adana, Turkey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yurdanur","family":"K\u0131l\u0131nc\u0327","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology-Oncology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaochuan","family":"Feng","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Glenda","family":"Batzer","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saul","family":"Tzipori","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Tufts University School of Veterinary Medicine, North Grafton, Massachusetts 01536"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanni","family":"Widmer","sequence":"additional","affiliation":[{"name":"Department of Pediatric Hematology-Oncology"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/0732-8893(94)90035-3"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004360050399"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(98)00119-4"},{"key":"e_1_3_2_5_2","first-page":"173","volume":"170","year":"1999","unstructured":"Cacci\u00f2, S., W. Homan, K. van Dijk, and E. Pozio. 1999. Genetic polymorphism at the beta-tubulin locus among human and animal isolates of Cryptosporidium parvum. FEMS Microbiol. Lett.170:173-179.","journal-title":"FEMS Microbiol. Lett."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.9.3958-3960.1997"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/173.1.232"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.4.3.325"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/aem.63.8.3134-3138.1997"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.8.3427-3432.1999"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0166-6851(01)00393-0"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.12.3255-3257.1992"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/1522-2683(200102)22:3<433::AID-ELPS433>3.0.CO;2-K"},{"key":"e_1_3_2_14_2","first-page":"38","volume":"40","year":"1998","unstructured":"Griffiths, J. K. 1998. Human cryptosporidiosis: epidemiology, transmission, clinical disease, treatment, and diagnosis. Adv. Parasitol.40:38-87.","journal-title":"Adv. Parasitol."},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.6.10.986"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(01)00339-6"},{"key":"e_1_3_2_17_2","unstructured":"Hogan B. F. Constantini and E. Lacy. 1986. Manipulating the mouse embryo: a laboratory manual. Cold Spring Harbor Laboratory Press Cold Spring Harbor N.Y."},{"key":"e_1_3_2_18_2","first-page":"3849","volume":"6","year":"1995","unstructured":"Johnson, D. W., N. J. Pieniazek, D. W. Griffin, L. Misener, and J. B. Rose. 1995. Development of a PCR protocol for sensitive detection of Cryptosporidium oocysts in water samples. Appl. Environ. Microbiol.6:3849-3855.","journal-title":"Appl. Environ. Microbiol."},{"key":"e_1_3_2_19_2","first-page":"341","year":"2001","unstructured":"Kr\u00fcger, P., A. Wiedenmann, D. Tougianidou, and K. Botzenhart. 2001. Quantitative detection of Cryptosporidium parvum after in vitro excystation by LightCycler PCR, p. 341-348. In S. Meyer, C. Witter, and K. I. Nakagawara (ed.), Rapid real-time PCR methods and applications. Springer-Verlag Berlin, Germany.","journal-title":"Rapid real-time PCR methods and applications"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/0166-6851(92)90155-D"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1991.45.688"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/147.5.824"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.3.1180-1183.2000"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1995.52.559"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.2307\/3285678"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1086\/315033"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.8.12.1299"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.68.1.427-429.2002"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1006\/abio.1996.9916"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.2.841-845.1999"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.2307\/3285725"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.65.10.4431-4435.1999"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/cdli.1.4.450-463.1994"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1159\/000060370"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.8.2013-2017.1997"},{"key":"e_1_3_2_36_2","first-page":"224","volume":"40","year":"1998","unstructured":"Widmer, G. 1998. Genetic heterogeneity and PCR detection of Cryptosporidium parvum. Adv. Parasitol.40:224-241.","journal-title":"Adv. Parasitol."},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.64.11.4477-4481.1998"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1086\/515373"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.66.12.5492-5498.2000"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1086\/318090"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.40.9.3237-3244.2002","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.40.9.3237-3244.2002","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T01:58:12Z","timestamp":1646445492000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.40.9.3237-3244.2002"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2002,9]]},"references-count":39,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2002,9]]}},"alternative-id":["10.1128\/JCM.40.9.3237-3244.2002"],"URL":"https:\/\/doi.org\/10.1128\/jcm.40.9.3237-3244.2002","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2002,9]]},"assertion":[{"value":"2002-04-10","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-06-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2002-09-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}